Computational Techniques Toward 1 nm Super-resolution Microscopy

This thesis explores advanced computational techniques in super-resolution microscopy (SRM), with the primary goal of pushing the limits of achievable resolution towards the 1 nm scale. It includes developments in particle fusion algorithms, data analysis of complex biological structures, and exploration of the impact of molecular dipole...

Microscopic 3D plant imaging with high-resolution optical coherence tomography

Towards labonachip optical trapping and Raman spectroscopy of extracellular vesicles using multiwaveguide traps

Optofluidic lab-on-a-chips (LOCs) employing a dual-waveguide trap for optical trapping and Raman spectroscopy have proven to be attractive and potent tools for high throughput chemical fingerprinting of bio-particles for disease diagnosis. Among the relevant bio-particles are extracellular vesicles (EVs) which a been proven through recent...

Localization microscopy of constrained fluorescent molecules: Pushing towards Ångström-scale resolution through cryogenics

<i>Localization microscopy has circumvented the diffraction limit by sequentially</i> imaging individual light emitting molecules at a time. The position of these individual molecules can be determined and a super-resolution reconstruction is made with improved resolution. Normally freely rotating emitters are used such that the point spread...

Operator-Based Modeling and Inversion: An Operator Approach to the Forward and Inverse Scattering Problems

The seismic method has many applications. It is important in the critical sector of energy. Besides being used in imaging oil and gas reservoirs, it is also utilized in other sectors of energy such as geothermal energy exploration and development. It also plays a role in extracting other resources such as minerals or in the process of monitoring...

Parameter estimation in single molecule microscopy

Computational optical imaging based on helical point spread functions

Helical point spread functions (PSFs) provide a powerful computational imaging tool for modern optical imaging and sensing applications. However, their utilization is, so far, limited to a single field of application, i.e. super-resolution microscopy, which is due to multiple shortcomings in their current system implementation. A new...

Multi-modal Whole Slide Imaging

This thesis explores a novel optical architecture for Whole Slide Imaging (WSI). This new architecture allows for multi-focal (3D) image acquisitions in a single scan pass. The multi-focal imaging capability is used to demonstrate 3D phase imaging and 3D imaging of thick tissue sections on a prototype scanner. Further, instrumentation for the...

Particle fusion in localization microscopy

Single molecule localization microscopy (SMLM) shows promise for quantitative structural analysis of subcellular complexes and organelles with a resolution well below the diffraction limit. This superresolution microscopy technique relies on the blinking events of fluorescent molecules that labeled the structure of interest and are...