Estimation of Drift in Localization Microscopy: A State Space Modelling Approach

Single Molecule Localization Microscopy (SMLM) has enabled researchers to breakthrough the diffraction limit and obtain nanometer resolution images of macromolecular structures. But due to the time involved in obtaining ample data for proper image, the technique is venerable to many problem including fluctuations due to thermal gradients from...

Modulation enhanced multi-emitter localization microscopy: Multi-emitter fitting with structured illumination patterns for high imaging density super-resolution localization microscopy

For a long time, scientists believed that it is not possible to achieve a better microscopic resolution than half the wavelength of light (~ 200 nm). During the last decades, various super-resolution fluorescence microscopy techniques have been developed, offering high-resolution imaging beyond the diffraction limit. It has been established as...

Data-Driven PSF Modeling using B-Splines

Super Resolution Microscopy is a technique to image objects with resolution higher than the diffraction limit, the fundamental limit to the resolution of a microscope. One of the techniques used for Super Resolution Microscopy is Single Molecule Localization Microscopy (SMLM). For this method, a sample is labeled using fluorophores (fluorescent...