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Oud, B. (author)Reverse engineering is the study of discovering the structure, function and operation of a device or system with the express aim to reconstruct its key functionalities. This principle is applied to many disciplines, from military, through computer engineering, to health, but also in metabolic engineering. In this context, reverse metabolic...doctoral thesis 2013
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- Romagnoli, G. (author) doctoral thesis 2014
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Solis Escalante, D. (author)Glycolysis, a biochemical pathway that oxidizes glucose to pyruvate, is at the core of sugar metabolism in Saccharomyces cerevisiae (bakers’ yeast). Glycolysis is not only a catabolic route involved in energy conservation, but also provides building blocks for anabolism. From an applied perspective, several glycolytic intermediates are key...doctoral thesis 2015
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Kozak, B.U. (author)Metabolic engineering – the improvement and addition, by genetic modification, of industrially relevant properties of microorganisms with respect to catalysis, transport and regulatory functions – is a well-established method for development of more cost-effective and ‘green’ industrial processes. Rapid depletion of oil reserves and a growing...doctoral thesis 2015
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- Hebly, M. (author) doctoral thesis 2015
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Bolat, I. (author)One of the best guarded secrets of brewers is represented by the brewing yeast employed in beer fermentation, due to its profound impact upon the specific flavour profile of the final product. The current research tackles the genome diversity of lager brewing strains as well as their impact on important phenotypic traits. Furthermore, the...doctoral thesis 2016
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- Bisschops, M.M.M. (author) doctoral thesis 2016
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Kuijpers, N.G.A. (author)In the past few decades, concerns on climate change have contributed to the development of biotechnological alternatives for petrochemical processes. In order to make these processes economically competitive, there has been an intensive search for improved, more efficient production organisms. In the past decade, genetic engineering of...doctoral thesis 2016
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- Milne, N.S.W. (author) doctoral thesis 2016
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Rossum, Harmen M. (author)CRISPR/Cas9 is a novel Swiss Army Knife in the field of synthetic biology. The thesis of Harmen van Rossum (Biotechnology/TNW) shows that this tool enables rapid genetic modification of baker’s yeast; six genetic changes could be introduced within one week – previously this could easily take a few months.<br/>The goal of the PhD project was to...doctoral thesis 2016
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- Vos, T. (author) doctoral thesis 2016
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Mans, R. (author)Microbial production of fuels and chemicals provides opportunities for replacing conventional production processes, which are based on chemical synthesis from non-renewable raw materials or on labour- and capital-intensive extraction from animal or plant tissues. Aeons of evolution, in which astronomical numbers of microorganisms competed for...doctoral thesis 2017
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Papapetridis, I. (author)Mankind’s energy requirements, which are currently mainly covered by the combustion of fossil fuels, have been steadily increasing in the past half century. While fossil fuels have a high energy content, their use results in significant emissions of greenhouse gases (mainly CO2, methane and nitrous oxide). As the industrialization of developing...doctoral thesis 2018
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Verhoeven, M.D. (author)The recent start-up of several full-scale ‘second generation’ ethanol plants marks a major milestone in the development of Saccharomyces cerevisiae yeast strains for fermentation of lignocellulosic hydrolysates of agricultural residues and energy crops. In contrast to the fermentation of hexose sugar-rich substrates, such as corn syrup or sugar...doctoral thesis 2018
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Marques, W.L. (author)Contemporary society heavily depends on fossil sources. The energy and materials derived from fossil reserves were major contributors to the acceleration and intensification of agriculture and industry over the past 100 years. Such reserves are finite, hence, after expanding geographically, our economy is now consuming natural reserves that...doctoral thesis 2018
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Bracher, J.M. (author)Nearly 200 parties signed and committed to the Paris agreement in 2017, which comprises the long-term goal to keep the average global temperature increase well below 2 degrees above pre-industrial levels. Virtually all possible scenarios drafted to reach this goal include a strongly increased use of biofuels for transport by land, sea and air....doctoral thesis 2019
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Brickwedde, A. (author)haploid S. cerevisiae laboratory strain of the CEN.PK family. The performance of the constructed hybrid, strain IMS0408, was then compared to those of its parents in anaerobic batch cultures grown on different media and at different temperatures. While S. eubayanus displayed significantly higher growth rates than S. cerevisiae in anaerobic batch...doctoral thesis 2019
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Gorter de Vries, A.R. (author)Lager beer, also referred to as Pilsner, is the most popular alcoholic beverage in the world, with an annual consumption of almost 200 billion litres per year. To make lager beer, brewer’s wort is fermented with the yeast <i style="mso-bidi-font-style:normal"...doctoral thesis 2019
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Hakkaart, X.D.V. (author)In industrial biotechnology, micro-organisms are used for making a wide range of products, including neutraceuticals, transport fuels, pharmaceuticals and plastics. The raw materials for these microbial processes are often sugars derived from natural resources. Industrial biotechnology offers an advantage with respect to the petrochemical...doctoral thesis 2019
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Valk, L.C. (author)Over the past decade the demand for technology which can make our global economy more sustainable has increased exponentially. In the field of biotechnology, one of the many advancements is focussed on the conversion of agricultural waste streams towards commodity chemicals. Plant biomass can be an environmental and sustainable alternative for...doctoral thesis 2020