Searched for: department%3A%22BT%255C%257Eslsh%257EBiotechnology%22
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Fernandez Fueyo, E. (author), Ruiz-Duenas, F.J. (author), Lopez-Lucendo, M.F. (author), Perez-Boada, M. (author), Rencorat, J. (author), Gutierrez, A. (author), Pisabarro, A.G. (author), Ramirez, L. (author), Martinez, A.T. (author)
Background: Pleurotus ostreatus is the second edible mushroom worldwide, and a model fungus for delignification applications, with the advantage of growing on woody and nonwoody feedstocks. Its sequenced genome is available, and this gave us the opportunity to perform proteomic studies to identify the enzymes overproduced in lignocellulose...
journal article 2016
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Van Rossum, H.M. (author), Kozak, B.U. (author), Pronk, J.T. (author), Van Maris, A.J.A. (author)
Saccharomyces cerevisiae is an important industrial cell factory and an attractive experimental model for evaluating novel metabolic engineering strategies. Many current and potential products of this yeast require acetyl coenzyme A (acetyl-CoA) as a precursor and pathways towards these products are generally expressed in its cytosol. The native...
journal article 2016
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van den Broeck, W. (author)
Fossil fuels will run out in the foreseeable future. Out entire economic system is build upon fossil fuels and building up another base for this economic system is challenging. The only alternative for making materials that normally are derived from fossil fuels is the Bio-Based Economy. Materials in this way get made from biomass, such as sugar...
master thesis 2016
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Prest, E.I.E.D. (author), Hammes, F. (author), Van Loosdrecht, M.C.M. (author), Vrouwenvelder, J.S. (author)
Biological stability of drinking water refers to the concept of providing consumers with drinking water of same microbial quality at the tap as produced at the water treatment facility. However, uncontrolled growth of bacteria can occur during distribution in water mains and premise plumbing, and can lead to hygienic (e.g., development of...
journal article 2016
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Suarez Mendez, C.A. (author), Hanemaaijer, M. (author), Ten Pierick, A. (author), Wolters, J.C. (author), Heijnen, J.J. (author), Wahl, S.A. (author)
13C labeling experiments in aerobic glucose limited cultures of Saccharomyces cerevisiae at four different growth rates (0.054; 0.101, 0.207, 0.307 h?1) are used for calculating fluxes that include intracellular cycles (e.g., storage carbohydrate cycles, exchange fluxes with amino acids), which are rearranged depending on the growth rate. At low...
journal article 2016
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Milne, N.S.W. (author), Wahl, S.A. (author), Van Maris, A.J.A. (author), Pronk, J.T. (author), Daran, J.M. (author)
It is theoretically possible to engineer Saccharomyces cerevisiae strains in which isobutanol is the predominant catabolic product and high-yielding isobutanol-producing strains are already reported by industry. Conversely, isobutanol yields of engineered S. cerevisiae strains reported in the scientific literature typically remain far below 10%...
journal article 2016
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De Almeida, N.M. (author), Neumann, S. (author), Mesman, R.J. (author), Ferousi, C. (author), Keltjens, J.T. (author), Jetten, M.S.M. (author), Kartal, B. (author), Van Niftrik, L. (author)
Anaerobic ammonium-oxidizing (anammox) bacteria oxidize ammonium with nitrite as the terminal electron acceptor to form dinitrogen gas in the absence of oxygen. Anammox bacteria have a compartmentalized cell plan with a central membrane-bound “prokaryotic organelle” called the anammoxosome. The anammoxosome occupies most of the cell volume, has...
journal article 2015
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Lin, Y. (author), Al-Zuhairy, S. (author), Pronk, M. (author), Van Loosdrecht, M.C.M. (author)
In a prior art reactor set up dense aggregates of microorganisms are formed, typically in or embedded in an extracellular matrix. Such may relate to granules, to sphere like entities having a higher viscosity than water, globules, a biofilm, etc. The dense aggregates comprise extracellular polymeric substances, or biopolymers, in particular...
patent 2015
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Schumacher, R. (author), Wahl, S.A. (author)
The design of microbial production processes relies on rational choices for metabolic engineering of the production host and the process conditions. These require a systematic and quantitative understanding of cellular regulation. Therefore, a novel method for dynamic flux identification using quantitative metabolomics and 13C labeling to...
journal article 2015
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Hagedoorn, P.L. (author)
Metalloproteomics is a rapidly developing field of science that involves the comprehensive analysis of all metal-containing or metal-binding proteins in a biological sample. The purpose of this review is to offer a comprehensive overview of the research involving approaches that can be categorized as inductively coupled plasma (ICP)-MS based...
journal article 2015
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Milne, N. (author), Van Maris, A.J.A. (author), Pronk, J.T. (author), Daran, J.M. (author)
Background: Decarboxylation of ?-ketoisovalerate to isobutyraldehyde is a key reaction in metabolic engineering of Saccharomyces cerevisiae for isobutanol production with published studies relying on overexpression of either the native ARO10 gene or of the Lactococcus lactis kivD decarboxylase gene resulting in low enzymatic activities. Here, we...
journal article 2015
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Hebly, M. (author)
doctoral thesis 2015
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Van Oosten, L.N. (author), Pieterse, M. (author), Pinkse, M.W.H. (author), Verhaert, P.D.E.M. (author)
Animal venoms and toxins are a valuable source of bioactive peptides with pharmacologic relevance as potential drug leads. A large subset of biologically active peptides discovered up till now contain disulfide bridges that enhance stability and activity. To discover new members of this class of peptides, we developed a workflow screening...
journal article 2015
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Honarmand Ebrahimi, K. (author), Hagedoorn, P.L. (author), Hagen, W.R. (author)
journal article 2015
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Saez-Jimenez, V. (author), Fernandez Fueyo, E. (author), Madrano, F.J. (author), Romero, A. (author), Martinez, A.T. (author), Ruiz-Duenas, F.J. (author)
Versatile peroxidase (VP) from the white-rot fungus Pleurotus eryngii is a high redox potential peroxidase of biotechnological interest able to oxidize a wide range of recalcitrant substrates including lignin, phenolic and non-phenolic aromatic compounds and dyes. However, the relatively low stability towards pH of this and other fungal...
journal article 2015
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Hiseni, A. (author), Otten, L.G. (author), Arends, I.W.C.E. (author)
Carotenoid 1,2-hydratases (CrtC) catalyze the selective addition of water to an isolated carbon–carbon double bond. Although their involvement in the carotenoid biosynthetic pathway is well understood, little is known about the mechanism by which these hydratases transform carotenoids such as lycopene into the corresponding hydroxyl compounds....
journal article 2015
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Daran, J.G. (author), Pronk, J.T. (author), Romagnoli, G. (author)
The invention relates to a nucleic acid molecule encoding a novel selection marker. Said marker is a guanidinobutyrase from Kluyveromyces lactis, which, when expressed in Saccharomyces, allows the growth of the yeast in the presence of guanidinobutyrate as the sole nitrogen source. Said marker can be used in a method for producing a...
patent 2015
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Junicke, H. (author), Van Loosdrecht, M.C.M. (author), Kleerebezem, R. (author)
Many anaerobic conversions proceed close to thermodynamic equilibrium and the microbial groups involved need to share their low energy budget to survive at the thermodynamic boundary of life. This study aimed to investigate the kinetic and thermodynamic control mechanisms of the electron transfer during syntrophic butyrate conversion in non...
journal article 2015
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Kleerebezem, R. (author), Joosse, B. (author), Rozendaal, R. (author), Van Loosdrecht, M.C.M. (author)
Anaerobic digestion for the production of methane containing biogas is the classic example of a resource recovery process that combines stabilization of particulate organic matter or wastewater treatment with the production of a valuable end-product. Attractive features of the process include the production of a single end-product from a...
journal article 2015
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Cuellar Soares, M.C. (author), Van der Wielen, L.A.M. (author), Heeres, A.S. (author)
The invention is directed to a method for recovering a lipid or hydrocarbon from a fermentation mixture, comprising the steps of - providing a fermentation mixture wherein the lipid or hydrocarbon is produced by microbial fermentation in a fermentation vessel, which mixture comprises an aqueous phase and a liquid product phase, wherein the...
patent 2015
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