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Veiga, T. (author), Gombert, A.K. (author), Landes, N. (author), Verhoeven, M.D. (author), Kiel, J.A.K.W. (author), Krikken, A. (author), Nijland, J.G. (author), Touw, H. (author), Luttik, M.A.H. (author), Van der Toorn, J.C. (author), Driessen, A.J.M. (author), Bovenberg, R.A.L. (author), Van den Berg, M.A. (author), Van der Klei, I.J. (author), Pronk, J.T. (author), Daran, J.M. (author)Industrial production of semi-synthetic cephalosporins by Penicillium chrysogenum requires supplementation of the growth media with the side-chain precursor adipic acid. In glucose-limited chemostat cultures of P. chrysogenum, up to 88% of the consumed adipic acid was not recovered in cephalosporin-related products, but used as an additional...journal article 2012
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Douma, R.D. (author), Batista, J.M. (author), Touw, K.M. (author), Kiel, J.A.K.W. (author), Krikken, A.M. (author), Zhao, Z. (author), Veiga, T. (author), Klaassen, P. (author), Bovenberg, R.A.L. (author), Daran, J.M. (author), Heijnen, J.J. (author), Van Gulik, W.M. (author)Background In microbial production of non-catabolic products such as antibiotics a loss of production capacity upon long-term cultivation (for example chemostat), a phenomenon called strain degeneration, is often observed. In this study a systems biology approach, monitoring changes from gene to produced flux, was used to study degeneration of...journal article 2011