Temperature impact on yeast metabolism: Insights from experimental and modeling approaches

Temperature is an environmental parameter that greatly affects the growth of microorganisms, due to its impact on the activity of all enzymes in the network. This is particularly relevant in habitats where there are large temperature changes, either daily or seasonal. Understanding how organisms have adapted to cope with these temperature-cycles...

Metabolic engineering of Saccharomyces cerevisiae for C4-dicarboxylic acid production

Biotechnological production of chemicals from renewable feedstocks offers a sustainable alternative to petrochemistry. Understanding of the biology of microorganisms and plants is increasing at an unprecedented rate and tools with which these organisms can be engineered for industrial application are becoming ever more powerful. However,...

Dynamic response of Saccharomyces cerevisiae to fermentative growth conditions

Growth and metabolism of Anammox Bacteria

The anoxic ammonium oxidation (anammox) process is the conversion of nitrite and ammonium under anoxic conditions- to form dinitrogen gas. The process is performed by deep-branching Planctomycetes. The startup of the first full-scale anammox reactor in the world is described in Chapter 2. The described full scale reactor was a granular sludge...

Development and application of 13C-labeling techniques: Analyzing the pentose phosphate pathway of Penicillium chrysogenum

The 13C-labeling technique is a powerful characterization tool within the field of metabolic engineering aimed at determining intracellular steady state fluxes. The provided metabolic snapshots enable researchers to better understand and predict the phenotypic behavior of a micro-organism as a result of genetic alterations and/or different...