Molybdenum incorporation in tungsten aldehyde oxidoreductase enzymes from Pyrococcus furiosus

The hyperthermophilic archaeon Pyrococcus furiosus expresses five aldehyde oxidoreductase (AOR) enzymes, all containing a tungsto-bispterin cofactor. The growth of this organism is fully dependent on the presence of tungsten in the growth medium. Previous studies have suggested that molybdenum is not incorporated in the active site of these...

A steady-state and pre-steady-state kinetics study of the tungstoenzyme formaldehyde ferredoxin oxidoreductase from Pyrococcus furiosus

Formaldehyde ferredoxin oxidoreductase from Pyrococcus furiosus is a homotetrameric protein with onetungstodipterin and one [4Fe–4S] cubane per 69-kDa subunit. The enzyme kinetics have been studied understeady-state conditions at 80 C and pre-steady state conditions at 50 C, in the latter case via monitoring of the relatively weak (e ~ 2 mM–1 cm...