Co-Culture of Precision-Cut Liver Slices and Liver-Derived Mesenchymal Stromal Cells: An ex vivo model for mesenchymal stromal cell administration during hypothermic oxygenated machine perfusion of the liver

Due to the limited availability of suitable livers for transplantation and the increasing use of suboptimal donor livers, which are more prone to ischemia-reperfusion injury (IRI), there is an urgent need for novel graft-ameliorating therapies. The promising capacities of (liver-derived) mesenchymal stromal cells ((L-)MSC) in mitigating IRI and...

Co-Culture of Liver-Derived Mesenchymal Stromal Cells and Intrahepatic Cholangiocyte Organoids in an Extracellular Matrix Environment: a Step Towards Complex Liver Models

To this day, liver transplantation is the only effective treatment for end-stage liver failure. Unfortunately, the scarcity of liver donors leads to waitlist mortality, pushing the need for alternative treatment options. In vitro models are essential tools in research on alternatives for transplantation. One of the promising models is the...

Towards Direct Co-culture Models for Osteoimmunodulatory Studies: An exploratory study on the effects of nanopatterns and shear stress on cellular crosstalk

In the last two decades, it has been generally accepted that the interaction between bone and immune cells is critical in the process of bone healing and regeneration. Steering this immune response with the development of biomaterials could lead to implant acceptance in the long term. The understanding of osteoimmunological responses is of high...