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Paul, C.E. (author), Eggerichs, Daniel (author), Westphal, Adrie H. (author), Tischler, Dirk (author), van Berkel, Willem J.H. (author)Flavoprotein monooxygenases (FPMOs) are single- or two-component enzymes that catalyze a diverse set of chemo-, regio- and enantioselective oxyfunctionalization reactions. In this review, we describe how FPMOs have evolved from model enzymes in mechanistic flavoprotein research to biotechnologically relevant catalysts that can be applied for...review 2021
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Qi, Jingxian (author), Paul, C.E. (author), Hollmann, F. (author), Tischler, Dirk (author)The oxygen-insensitive azoreductase AzoRo originating from Rhodococcus opacus 1CP was found to be most active at low pH (ca. 4) and high temperature (ca. 50 °C). AzoRo is not an efficient biocatalyst when used at low pH due to stability problems. To overcome this issue, we discovered that AzoRo accepts an alternative electron donor, 1-benzyl...journal article 2017