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Phenolic and petrochemical wastewater treatment in AnMBR under extreme conditions: High salinity, high temperature, and high concentration of toxic compounds

Garcia Rea, V.S. (author)

Anaerobic digestion (AD) is a biochemical process in which organic matter is converted into biogas in a series of biochemical reactions. The development of high-rate anaerobic reactors (HRAR) led to the breakthrough of full-scale applications of AD for the treatment of industrial wastewater. HRARs, such as the upflow anaerobic sludge blanket ...

doctoral thesis 2023

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Mixed-Culture Anaerobic Acidogenic Fermentation of Cabbage: Decoding Community Selection

Christofi, Andreas (author)

Dating back to 10,000 B.C.E., fermentation has served as a vital tool primarily for food preservation. In the nineteenth century, Louis Pasteur put forth the proposition that the occurrence of fermentation is predominantly facilitated by the presence of microorganisms. Pasteur’s discovery spurred comprehensive studies on microorganisms,...

master thesis 2023

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Broadband EPR Spectroscopy of the Triplet State: Multi-Frequency Analysis of Copper Acetate Monohydrate

Hagen, W.R. (author)

Electron paramagnetic resonance spectroscopy is a long-standing method for the exploration of electronic structures of transition ion complexes. The difficulty of its analysis varies considerably, not only with the nature of the spin system, but more so with the relative magnitudes of the magnetic interactions to which the spin is subject, where...

journal article 2023

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Quantification and mitigation of byproduct formation by low-glycerol-producing Saccharomyces cerevisiae strains containing Calvin-cycle enzymes

van Aalst, A.C.A. (author), Jansen, Mickel L.A. (author), Mans, R. (author), Pronk, J.T. (author)

Background: Anaerobic Saccharomyces cerevisiae cultures require glycerol formation to re-oxidize NADH formed in biosynthetic processes. Introduction of the Calvin-cycle enzymes phosphoribulokinase (PRK) and ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) has been shown to couple re-oxidation of biosynthetic NADH to ethanol...

journal article 2023

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Electroreduction of Carbon Dioxide to Acetate using Heterogenized Hydrophilic Manganese Porphyrins

Abdinejad, M. (author), Yuan, T. (author), Tang, Keith (author), Duangdangchote, Salatan (author), Iglesias van Montfort, H.P. (author), Li, Mengran (author), Middelkoop, J. (author), Wolff, M.J. (author), Burdyny, T.E. (author)

The electrochemical reduction of carbon dioxide (CO₂) to value-added chemicals is a promising strategy to mitigate climate change. Metalloporphyrins have been used as a promising class of stable and tunable catalysts for the electrochemical reduction reaction of CO₂ (CO₂RR) but have been primarily restricted...

journal article 2023

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Acetic acid, growth rate, and mass transfer govern shifts in CO metabolism of Clostridium autoethanogenum

Perdigão Elisiário, M. (author), Van Hecke, Wouter (author), De Wever, Heleen (author), Noorman, H.J. (author), Straathof, Adrie J.J. (author)

Abstract: Syngas fermentation is a leading microbial process for the conversion of carbon monoxide, carbon dioxide, and hydrogen to valuable biochemicals. Clostridium autoethanogenum stands as a model organism for this process, showcasing its ability to convert syngas into ethanol industrially with simultaneous fixation of carbon and...

journal article 2023

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Co-cultivation of Saccharomyces cerevisiae strains combines advantages of different metabolic engineering strategies for improved ethanol yield

van Aalst, A.C.A. (author), van der Meulen, Igor S. (author), Jansen, Mickel L.A. (author), Mans, R. (author), Pronk, J.T. (author)

Glycerol is the major organic byproduct of industrial ethanol production with the yeast Saccharomyces cerevisiae. Improved ethanol yields have been achieved with engineered S. cerevisiae strains in which heterologous pathways replace glycerol formation as the predominant mechanism for anaerobic re-oxidation of surplus NADH generated in...

journal article 2023

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Optimizing the balance between heterologous acetate- and CO2-reduction pathways in anaerobic cultures of Saccharomyces cerevisiae strains engineered for low-glycerol production

van Aalst, Aafke C.A. (author), Geraats, Ellen H. (author), Martini, M.L.A. (author), Mans, R. (author), Pronk, J.T. (author)

In anaerobic Saccharomyces cerevisiae cultures, NADH (reduced form of nicotinamide adenine dinucleotide)-cofactor balancing by glycerol formation constrains ethanol yields. Introduction of an acetate-to-ethanol reduction pathway based on heterologous acetylating acetaldehyde dehydrogenase (A-ALD) can replace glycerol formation as 'redox-sink'...

journal article 2023

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Calcium enhances polyhydroxyalkanoate production and promotes selective growth of the polyhydroxyalkanoate-storing biomass in municipal activated sludge

Estevez Alonso, A. (author), Arias-Buendía, María (author), Pei, R. (author), van Veelen, H. Pieter J. (author), van Loosdrecht, Mark C.M. (author), Kleerebezem, R. (author), Werker, A. (author)

Activated sludge from municipal wastewater treatment processes can be used directly for the production of biodegradable polyesters from the family of polyhydroxyalkanoates (PHAs). However, municipal activated sludge typically cannot accumulate PHAs to very high levels and often low yields of polymer produced on substrate are observed. In the...

journal article 2022

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Enhancing the Separation Efficiency in Acetic Acid Manufacturing by Methanol Carbonylation

Dimian, Alexandre C. (author), Kiss, A.A. (author)

Acetic acid is an essential chemical used in the production of many chemical products. Using methanol as bio-building block, the acetic acid produced may be incorporated in bio-based products. This study deals with enhancing the efficiency of separations in a process using a homogeneous catalyst, which is the most applied today industrially....

journal article 2021

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From Scratch Closure to Electrolyte Barrier Restoration in Self-Healing Polyurethane Coatings

Montano, V. (author), Vogel, Wouter (author), Smits, Angela (author), van der Zwaag, S. (author), Garcia, Santiago J. (author)

The effects of the soft block fraction and H-bond state in thermoplastic polyurethanes on autonomous entropy-driven scratch closure and barrier restoration are studied. To this aim, comparable polyurethanes with different segmentation states are applied as organic coatings on plain carbon steel plates, scratched under very well-controlled...

journal article 2021

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Catalytic reforming of acetic acid as main primary tar compound from biomass updraft gasifiers: screening of suitable catalysts and operating conditions

Cavalli, A. (author), Tetteroo, R. (author), Graziadio, M. (author), Aravind, P.V. (author)

Tar compounds have been defined as Achilles’ heel in biomass gasification. Catalytic reforming solves problems caused by tar by converting them into H₂ and CO. Most of the research has focused on secondary and tertiary tar reforming while some information on primary tar can be derived from bio-oil reforming. However, these studies...

journal article 2021

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Enhancing Phenol Conversion Rates in Saline Anaerobic Membrane Bioreactor Using Acetate and Butyrate as Additional Carbon and Energy Sources

Garcia Rea, V.S. (author), Muñoz Sierra, Julian (author), Fonseca Aponte, L.M. (author), Cerqueda-Garcia, Daniel (author), Mollamohseni Quchani, K. (author), Spanjers, H. (author), van Lier, J.B. (author)

Phenolic industrial wastewater, such as those from coal gasification, are considered a challenge for conventional anaerobic wastewater treatment systems because of its extreme characteristics such as presence of recalcitrant compounds, high toxicity, and salinity. However, anaerobic membrane bioreactors (AnMBRs) are considered of potential...

journal article 2020

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A traffic light enzyme: acetate binding reversibly switches chlorite dismutase from a red- to a green-colored heme protein

Mahor, D. (author), Puschmann, J. (author), van den Haak, Menno (author), Kooij, Pepijn J. (author), van den Ouden, David L.J. (author), Strampraad, M.J.F. (author), Srour, B. (author), Hagedoorn, P.L. (author)

Abstract: Chlorite dismutase is a unique heme enzyme that catalyzes the conversion of chlorite to chloride and molecular oxygen. The enzyme is highly specific for chlorite but has been known to bind several anionic and neutral ligands to the heme iron. In a pH study, the enzyme changed color from red to green in acetate buffer pH 5.0. The...

journal article 2020

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Subcritical CO₂ shows no effect on liquid hot water pretreatment of poplar wood

Jimenez Gutierrez, J.M. (author), van der Wielen, L.A.M. (author), Straathof, Adrie J.J. (author)

Pretreatment of lignocellulosic biomass is required for many biorefinery processes. Previous studies have described hydrolysis of hemicelluloses by using liquid hot water (LHW) pretreatment. We evaluated the effect of carbonic acid originating from pressurized carbon dioxide during LHW pretreatment of poplar. The conditions applied covered...

journal article 2020

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Recovery of acetate by anion exchange with consecutive CO₂-expanded methanol desorption: A model-based approach

Cabrera Rodriguez, C.I. (author), Cartin Caballero, C.M. (author), Platarou, E. (author), de Weerd, F.A. (author), van der Wielen, L.A.M. (author), Straathof, Adrie J.J. (author)

Production of bio-based acetate is commonly hindered by the high costs of the downstream processing. In this paper, a model is developed to describe a new method that recovers acetate salts using anion exchange resins, and subsequently desorbs and upgrades them using CO₂-expanded alcohol. The model consists of equilibrium...

journal article 2018

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Metabolism and occurrence of methanogenic and sulfate-reducing syntrophic acetate oxidizing communities in haloalkaline environments

Timmers, Peer H.A. (author), Vavourakis, Charlotte D. (author), Kleerebezem, R. (author), Sinninghe Damsté, Jaap S. (author), Muyzer, Gerard (author), Stams, Alfons J.M. (author), Sorokin, Dimitry Y. (author), Plugge, Caroline M. (author)

Anaerobic syntrophic acetate oxidation (SAO) is a thermodynamically unfavorable process involving a syntrophic acetate oxidizing bacterium (SAOB) that forms interspecies electron carriers (IECs). These IECs are consumed by syntrophic partners, typically hydrogenotrophic methanogenic archaea or sulfate reducing bacteria. In this work, the...

journal article 2018

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Selection of Nutrient Used in Biogenic Healing Agent for Cementitious Materials

Tziviloglou, E. (author), Wiktor, V.A.C. (author), Jonkers, H.M. (author), Schlangen, E. (author)

Biogenic self-healing cementitious materials target on the closure of micro-cracks with precipitated inorganic minerals originating from bacterial metabolic activity. Dormant bacterial spores and organic mineral compounds often constitute a biogenic healing agent. The current paper focuses on the investigation of the most appropriate organic...

journal article 2017

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Metabolic engineering strategies for optimizing acetate reduction, ethanol yield and osmotolerance in Saccharomyces cerevisiae

Papapetridis, I. (author), van Dijk, M. (author), van Maris, A.J.A. (author), Pronk, J.T. (author)

Background: Glycerol, whose formation contributes to cellular redox balancing and osmoregulation in Saccharomyces cerevisiae, is an important by-product of yeast-based bioethanol production. Replacing the glycerol pathway by an engineered pathway for NAD⁺-dependent acetate reduction has been shown to improve ethanol yields and...

journal article 2017

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A new laboratory evolution approach to select for constitutive acetic acid tolerance in Saccharomyces cerevisiae and identification of causal mutations

Gonzalez Ramos, D. (author), Gorter de Vries, A.R. (author), Grijseels, Sietske S. (author), van Berkum, M.C. (author), Swinnen, Steve (author), van den Broek, M.A. (author), Nevoigt, Elke (author), Daran, J.G. (author), Pronk, J.T. (author), van Maris, A.J.A. (author)

Background: Acetic acid, released during hydrolysis of lignocellulosic feedstocks for second generation bioethanol production, inhibits yeast growth and alcoholic fermentation. Yeast biomass generated in a propagation step that precedes ethanol production should therefore express a high and constitutive level of acetic acid tolerance before...

journal article 2016