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Hülsen, Tim (author), Venturato, Daniel (author), Chan, Clement (author), Vandi, Luigi (author), Laycock, Bronwyn (author), Pratt, Steven (author), Stegman, Samuel (author), van Loosdrecht, Mark C.M. (author), Batstone, Damien J. (author)Anoxygenic purple phototrophic bacteria (PPB) were utilised in an 80 L biofilm flat plate photobioreactor to generate polyhydroxyalkanoate (PHA) over 44 cycles, with acetate as feed. Over the cycles, net PHA yield (growth + accumulation) averaged 21% while accumulation yield averaged 55%. Average PHA content was 35 wt% volatile solids (VS),...journal article 2024
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Gao, Yu (author), Zhang, Jingru (author), Wang, Donglei (author), Fan, Jiaxin (author), Mol, J.M.C. (author), Wang, Fuhui (author), Zhang, Danni (author), Xu, Dake (author)Microbially influenced corrosion (MIC) of metals exerts a negative effect on the marine environment and causes a great loss of marine facilities. Corrosion prevention in an eco-friendly and sustainable way is a difficult problem to address, especially in the marine environment. In this work, Nocardiopsis dassonville, a corrosive bacteria...journal article 2024
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Quispe Haro, Juan José (author), Chen, Fei (author), Los, R. (author), Shi, Shuqi (author), Sun, Wenjun (author), Chen, Yong (author), Idema, T. (author), Wegner, Seraphine V. (author)The transition of bacteria from an individualistic to a biofilm lifestyle profoundly alters their biology. During biofilm development, the bacterial cell-cell adhesions are a major determinant of initial microcolonies, which serve as kernels for the subsequent microscopic and mesoscopic structure of the biofilm, and determine the resulting...journal article 2024
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Fang, Jiaxing (author), Dai, Zihan (author), Li, Xiaoming (author), van der Hoek, J.P. (author), Savić, Dragan (author), Medema, G.J. (author), van der Meer, Walter (author), Liu, G. (author)Biofilm detachment contributes to water quality deterioration. However, the contributions of biofilm detachment from different pipes have not been quantified or compared. Following the introduction of partial reverse osmosis (RO) in drinking water production, this study analyzed particles at customers’ ends and tracked their origins to water...journal article 2023
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Trikannad, S.A. (author), van Halem, D. (author), Foppen, J.W.A. (author), van der Hoek, J.P. (author)Slow Sand Filtration is popular in drinking water treatment for the removal of a wide range of contaminants (e.g., particles, organic matter, and microorganisms). The Schmutzdecke in slow sand filters (SSFs) is known to be essential for pathogen removal, however, this layer is also responsible for increased head loss. Since the role of deeper...journal article 2023
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Fitriani, Nurina (author), Theresia, Ledy (author), O'Marga, Timothy Tjahja Nugraha (author), Kurniawan, Setyo Budi (author), Supriyanto, Agus (author), Abdullah, Siti Rozaimah Sheikh (author), Rietveld, L.C. (author)The present study investigated the utilization of blood clam shells as a potential substitute for conventional media, as well as the influence of the acclimation time on the efficacy of an intermittent slow sand filter (ISSF) in the treatment of real domestic wastewater. ISSF was operated with 16 h on and 8 h off, focusing on the parameters...journal article 2023
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Chen, L.M. (author), de Bruin, S. (author), Pronk, M. (author), Sousa, Diana Z. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)Anaerobic and aerobic granular sludge processes are widely applied in wastewater treatment. In these systems, microorganisms grow in dense aggregates due to the production of extracellular polymeric substances (EPS). This study investigates the sialylation and sulfation of anionic glyconconjugates in anaerobic and aerobic granular sludges...journal article 2023
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Ahmadi, Navid (author), Abbasi, Mona (author), Torabian, Ali (author), van Loosdrecht, Mark C.M. (author), Ducoste, Joel (author)We investigated the transformation of four pharmaceuticals (Diclofenac, Naproxen, Ibuprofen and Carbamazepine) in a moving bed biofilm reactor subjected to different COD/N ratios in four experimental phases. The shift from medium to high range COD/N ratio (i.e., 5:1 to 100:1) intensified the competition between heterotrophs and nitrifying...journal article 2023
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Tomas Martinez, S. (author), Zwolsman, Erwin J. (author), Merlier, Franck (author), Pabst, Martin (author), Lin, Y. (author), van Loosdrecht, Mark C.M. (author), Weissbrodt, D.G. (author)Polyphosphate accumulating organisms (PAOs) are responsible for enhanced biological phosphate removal (EBPR) from wastewater, where they grow embedded in a matrix of extracellular polymeric substances (EPS). EPSs comprise a mixture of biopolymers like polysaccharides or (glyco)proteins. Despite previous studies, little is known about the...journal article 2023
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Winkelhorst, O.M. (author), Cabau Peinado, O. (author), Straathof, Adrie J.J. (author), Jourdin, L. (author)Microbial electrochemical technologies (METs) employ microorganisms utilizing solid-state electrodes as either electron sink or electron source, such as in microbial electrosynthesis (MES). METs reaction rate is traditionally normalized to the electrode dimensions or to the electrolyte volume, but should also be normalized to biomass amount...journal article 2023
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Ahmad, J.I. (author)Drinking water distribution systems (DWDSs) are intended to supply hygienically safe and biostable water for human consumption. To supply aesthetically pleasant drinking water at the customers tap, water treatment and supply requires energy for production and distribution purposes (e.g. overall between 0.47 kWh/m<sup>3</sup> in the Netherlands)....doctoral thesis 2022
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van den Berg, L. (author), Toja Ortega, S. (author), van Loosdrecht, Mark C.M. (author), de Kreuk, M.K. (author)Aerobic granular sludge (AGS) is an advanced biofilm-based technology for wastewater treatment. Diffusion of substrates into the granules is a key aspect of this technology. Domestic wastewater contains soluble organic substrates of different sizes that could potentially diffuse into the granules. In this study, the relation between the...journal article 2022
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Desmond, Peter (author), Huisman, Kees Theo (author), Sanawar, Huma (author), Farhat, Nadia M. (author), Traber, Jacqueline (author), Fridjonsson, Einar O. (author), Johns, Michael L. (author), Flemming, Hans Curt (author), Picioreanu, C. (author), Vrouwenvelder, J.S. (author)The application of membrane technology for water treatment and reuse is hampered by the development of a microbial biofilm. Biofilm growth in micro-and ultrafiltration (MF/UF) membrane modules, on both the membrane surface and feed spacer, can form a secondary membrane and exert resistance to permeation and crossflow, increasing energy demand...journal article 2022
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Javier, Luisa (author), Pulido-Beltran, Laura (author), Vrouwenvelder, J.S. (author), Farhat, Nadia M. (author)Nutrient limitation has been proposed as a biofouling control strategy for membrane systems. However, the impact of permeation on biofilm development under phosphorus-limited and enriched conditions is poorly understood. This study analyzed biofilm development in membrane fouling simulators (MFSs) with and without permeation supplied with...journal article 2022
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Rivera, Emilio S. (author), Weiss, Andy (author), Migas, L.G. (author), Freiberg, Jeffrey A. (author), Djambazova, Katerina V. (author), Neumann, Elizabeth K. (author), Van de Plas, Raf (author), Spraggins, Jeffrey M. (author), Skaar, Eric P. (author), Caprioli, Richard M. (author)Introduction: Although Staphylococcus aureus is the leading cause of biofilm-related infections, the lipidomic distributions within these biofilms is poorly understood. Here, lipidomic mapping of S. aureus biofilm cross-sections was performed to investigate heterogeneity between horizontal biofilm layers. Methods: S. aureus biofilms were...journal article 2022
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Lattwein, K.R. (author), Beekers, Inés (author), Kouijzer, Joop J.P. (author), Leon-Grooters, Mariël (author), Langeveld, Simone A.G. (author), van Rooij, Tom (author), van der Steen, A.F.W. (author), de Jong, N. (author), van Wamel, Willem J.B. (author), Kooiman, Klazina (author)Bacteria encased in a biofilm poses significant challenges to successful treatment, since both the immune system and antibiotics are ineffective. Sonobactericide, which uses ultrasound and microbubbles, is a potential new strategy for increasing antimicrobial effectiveness or directly killing bacteria. Several studies suggest that...journal article 2022
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Pirzadian, J. (author), Souhoka, T. (author), Herweijer, M. (author), van Heel, M.E. (author), van Wamel, W. J.B. (author), Goossens, R.H.M. (author), Severin, J. A. (author), Vos, M. C. (author)In hospitals, sinks act as reservoirs for bacterial pathogens. To assess the extent of splashing, fluorescein dye was added to four hospital sinks previously involved in pathogen dispersal to the environment and/or transmission to patients, and one sink that was not. Applying dye to the p-trap or tailpiece did not result in any fluorescent...journal article 2022
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Veeger, M.I.A. (author), Prieto Hoces, A.I. (author), Ottele, M. (author)A bioreceptive material allows for biological content (biofilms) to grow on it, without necessarily affecting the material itself. If a bioreceptive concrete could therefore be integrated into a building façade, it could lead to green façades that do not need additional technical systems. As part of previous research by the authors, a promising...journal article 2021
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Balasubramanian, S. (author), Yu, K. (author), Vasquez Cardenas, D. (author), Aubin-Tam, M.E. (author), Meyer, Anne S. (author)Biofilms are three-dimensional (3D) bacterial communities that exhibit a highly self-organized nature in terms of their composition and complex architecture. Bacteria in biofilms display emergent biological properties, such as resistance to antimicrobials and disinfectants that the individual planktonic cells lack. Bacterial biofilms possess...journal article 2021
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Perdigão Elisiário, M. (author), De Wever, Heleen (author), Van Hecke, Wouter (author), Noorman, H.J. (author), Straathof, Adrie J.J. (author)Syngas fermentation to biofuels and chemicals is an emerging technology in the biobased economy. Mass transfer is usually limiting the syngas fermentation rate, due to the low aqueous solubilities of the gaseous substrates. Membrane bioreactors, as efficient gas–liquid contactors, are a promising configuration for overcoming this gas-to...review 2021