Draft genome sequence of the Saccharomyces cerevisiae SpyCas9 expressing strain IMX2600, a laboratory and platform strain from the CEN.PK lineage for cell-factory research

The biobased-economy aims to create a circular biotechnology ecosystem to transition from a fossil fuel-based to a sustainable industry based on biomass. For this, new microbial cell-factories are essential. We present the draft genome of the CEN.PK-derived Saccharomyces cerevisiae SpyCas9 expressing strain (IMX2600), that serve as chassis of...

Towards fermentation of galacturonic acid-containing feedstocks with Saccharomyces cerevisiae

The ambition to reduce our current dependence on fossil transportation fuels has driven renewed interest in bioethanol. Pectin-rich feedstocks like sugar beet pulp and citrus peel, which are currently sold as cattle feed, are promising raw materials for the production of bioethanol. This thesis explores the challenges related to the fermentation...

Metabolic engineering of free-energy (ATP) conserving reactions in Saccharomyces cerevisiae

Metabolic engineering – the improvement of cellular activities by manipulation of enzymatic, transport and regulatory functions of the cell – has enabled the industrial production of a wide variety of biological molecules from renewable resources. Microbial production of fuels and chemicals thereby provides an alternative to oil-based production...