Flexural performance of squared one-sided CFRP patches: modelling and experimental study

Three-point bending analysis, experimental and numerical, is conducted for three different sizes, 30*30 mm, 40*40 mm and 60*60 mm, of one-sided square cross-ply TEXIPREG HS 160 RM carbon-fiber-reinforced polymer (CFRP) patches as well as intact and notched specimens. The tests are carried out with the aim to study the influence of the patch’s...

Flavoprotein monooxygenases: Versatile biocatalysts

Flavoprotein monooxygenases (FPMOs) are single- or two-component enzymes that catalyze a diverse set of chemo-, regio- and enantioselective oxyfunctionalization reactions. In this review, we describe how FPMOs have evolved from model enzymes in mechanistic flavoprotein research to biotechnologically relevant catalysts that can be applied for...

Biocatalytic Aromaticity-Breaking Epoxidation of Naphthalene and Nucleophilic Ring-Opening Reactions

Aromatic hydroxylation reactions catalyzed by heme-thiolate enzymes proceed via an epoxide intermediate. These aromatic epoxides could be valuable building blocks for organic synthesis giving access to a range of chiral trans-disubstituted cyclohexadiene synthons. Here, we show that naphthalene epoxides generated by fungal peroxygenases can...

Enhanced Interface Adhesion by Novel Eco-Epoxy Adhesives Based on the Modified Tannic Acid on Al and CFRP Adherends

This paper presents a new process for obtaining eco-epoxide adhesives synthesized from bio-renewable raw material (tannic acid-TA) and used for bonding lightweight materials (aluminum (Al) and carbon fiber reinforced polymer (CFRP). Two synthesized bio-epoxy components based on TA, (A) glycidyl ether and (B) glycidyl phosphate ester of TA,...

An Efficient Strategy for the Production of Epoxidized Oils: Natural Deep Eutectic Solvent-Based Enzymatic Epoxidation

Poor H ₂ O ₂ -resistance by enzymes is a key bottleneck in the epoxidation process of oil by enzymatic methods. In this study, the stability of three lipases, from Aspergillus oryzae lipase (AOL), Aspergillus fumigatus lipase B (AflB), and marine Janibacter (MAJ1), in the presence of H ₂ O ₂ was...

How To Break the Janus Effect of H ₂ O ₂ in Biocatalysis? Understanding Inactivation Mechanisms To Generate more Robust Enzymes

H ₂ O ₂ , is an attractive oxidant for synthetic chemistry, especially if activated as percarboxylic acid. H ₂ O ₂ , however, is also a potent inactivator of enzymes. Protein engineering efforts to improve enzyme resistance against H ₂ O ₂ in the past have mostly focused on...

P450BM3-Catalyzed oxidations employing dual functional small molecules

A set of dual functional small molecules (DFSMs) containing different amino acids has been synthesized and employed together with three different variants of the cytochrome P450 monooxygenase P450BM3 from Bacillus megaterium in H2O2-dependent oxidation reactions. These DFSMs enhance P450BM3 activity with hydrogen peroxide as an oxidant,...

Peroxygenase-Catalysed Epoxidation of Styrene Derivatives in Neat Reaction Media

Biocatalytic oxyfunctionalisation reactions are traditionally conducted in aqueous media limiting their production yield. Here we report the application of a peroxygenase in neat reaction conditions reaching product concentrations of up to 360 mM.

Deep Eutectic Solvents Enable More Robust Chemoenzymatic Epoxidation Reactions

A chemoenzymatic method for the production of epoxidized vegetable oils was developed. The unique combination of the commercial lipase G from Penicillieum camembertii with certain deep eutectic solvents enabled the efficient production of epoxidized vegetable oils.

Novel approaches for biocatalytic oxyfunctionalization reactions

Biocatalytic oxyfunctionalizations, especially of non - activated hydrocarbons, attract considerable attention for synthetic organic chemistry thanks to their high chemo-, regio-, and stereoselectivity, which difficult to achieve by chemical means. Many enzymatic oxyfunctionalizations have been described. However, there are many hurdles towards...

Iodine and Lipase Green Oxidation Technology

An exploration of Silsesquioxanes and Zeolites using High-Speed experimentation

Combinatorial Chemistry and High-Speed Experimentation techniques allow the rapid preparation and testing of large numbers of samples by using automated workstations. These techniques are increasingly applied to various fields of chemical research and particularly to catalysis. In this project, High-Speed Experimentation techniques were used to...