Fluorescence imaging for whole slide scanning using LED-based color sequential illumination

In the field of pathology there is an ongoing transition to the use of Whole Slide Imaging (WSI) systems which scan tissue slides at intermediate resolution (0∼.25 μm) and high throughput (15mm²=min) to digital image files. Most scanners currently on the market are line-sensor based push-broom scanners for three-color (RGB)...

Shack-Hartmann sensor based optical quality testing of whole slide imaging systems for digital pathology

Whole Slide Imaging (WSI) systems are used in the emerging field of digital pathology for capturing high-resolution images of tissue slides at high throughput. We present a technique to measure the optical aberrations of WSI systems using a Shack-Hartmann wavefront sensor as a function of field position. The resulting full-field aberration maps...