Beyond Saccharomyces pastorianus for modern lager brews: Exploring non-cerevisiae Saccharomyces hybrids with heterotic maltotriose consumption and novel aroma profile

Non-domesticated, wild Saccharomyces yeasts have promising characteristics for beer diversification, particularly when used in the generation of de novo interspecific hybrids. A major motivation for the current work was the question whether attractive novel Saccharomyces interspecific hybrids can be created for the production of exotic lager...

Improving Industrially Relevant Phenotypic Traits by Engineering Chromosome Copy Number in Saccharomyces pastorianus

The lager-brewing yeast Saccharomyces pastorianus is a hybrid between S. cerevisiae and S. eubayanus with an exceptional degree of aneuploidy. While chromosome copy number variation (CCNV) is present in many industrial Saccharomyces strains and has been linked to various industrially-relevant traits, its impact on the brewing performance of S...

Chromosome level assembly and comparative genome analysis confirm lager-brewing yeasts originated from a single hybridization

Background: The lager brewing yeast, S. pastorianus, is a hybrid between S. cerevisiae and S. eubayanus with extensive chromosome aneuploidy. S. pastorianus is subdivided into Group 1 and Group 2 strains, where Group 2 strains have higher copy number and a larger degree of heterozygosity for S. cerevisiae chromosomes. As a result, Group 2...

Phenotype-independent isolation of interspecies Saccharomyces hybrids by dual-dye fluorescent staining and fluorescence-activated cell sorting

Interspecies hybrids of Saccharomyces species are found in a variety of industrial environments and often outperform their parental strains in industrial fermentation processes. Interspecies hybridization is therefore increasingly considered as an approach for improvement and diversification of yeast strains for industrial application....

Analysis of the hybrid genomes of brewing yeasts

One of the best guarded secrets of brewers is represented by the brewing yeast employed in beer fermentation, due to its profound impact upon the specific flavour profile of the final product. The current research tackles the genome diversity of lager brewing strains as well as their impact on important phenotypic traits. Furthermore, the...

Betaalbare woningen voor de lagere inkomensgroep in Suriname

The urban design and the housing designs gives an answer to affordable living, while preserving and even improving the comfort of the house and neighbourhood. The urban plan and housing designs are not only applicable on the project location Richelieu, but it can also be applied in other districts of Suriname. It can be used as a tool to create...

Het ontwerp van elektromagneten, spindel en het frame in een zwevende spindelopstelling

Actieve magnetische lagering (AML) is het in positie houden van een roterende spindel. In deze thesis wordt het ontwerp van enkele onderdelen van een vereenvoudigde AML-opstelling uiteen gezet. Het verschil met een conventionele AML-opstelling is dat de spindel slechts in tweedimensionale richting wordt gestabiliseerd en deze niet roteert. De...