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Nutter, John (author), Qi, Jiahui (author), Farahani, H. (author), Rainforth, W. Mark (author), van der Zwaag, S. (author)The current study reports in situ TEM observations of the growth of bainitic ferrite in an Fe-0.3C-3Mn-1.5Si-0.15Mo steel held isothermally at 300 °C with a higher spatio-temporal resolution than in previous studies. Significant variations were found in the lengthening rate, with the highest being in excess of 30,000 nm.s<sup>−1</sup> while...journal article 2023
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Yang, Sora (author), Klughammer, N. (author), Barth, A. (author), Tanenbaum, M. (author), Dekker, C. (author)Single-molecule fluorescence imaging experiments generally require sub-nanomolar protein concentrations to isolate single protein molecules, which makes such experiments challenging in live cells due to high intracellular protein concentrations. Here, we show that single-molecule observations can be achieved in live cells through a drastic...journal article 2023
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Bauer, T.A. (author), Davis, Timothy J. (author), Frank, Bettina (author), Dreher, Pascal (author), Janoschka, David (author), Meiler, Tim C. (author), Meyer zu Heringdorf, Frank J. (author), Kuipers, L. (author), Giessen, Harald (author)The creation and manipulation of optical vortices, both in free space and in two-dimensional systems such as surface plasmon polaritons (SPPs), has attracted widespread attention in nano-optics due to their robust topological structure. Coupled with strong spatial confinement in the case of SPPs, these plasmonic vortices and their underlying...journal article 2023
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de Rooij, Jimmy (author), Vintges, M.Q. (author), Zuidwijk, T. (author), Heerkens, C.T.H. (author), Schulp, Anne S. (author)Identification of ontogenetic age classes plays an important role in the fields of zoology, palaeontology and archaeology, where accurate age classifications of (sub)fossil remains are a crucial component for the reconstruction of past life. Textural ageing—the identification of age-related bone surface textures—provides a size-independent...journal article 2023
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Rafigh Doost, J. (author), Kolenov, D. (author), Pereira, S.F. (author)It has been a widely growing interest in using silicon carbide (SiC) in high-power electronic devices. Yet, SiC wafers may contain killer defects that could reduce fabrication yield and make the device fall into unexpected failures. To prevent these failures from happening, it is very important to develop inspection tools that can detect,...journal article 2023
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Noom, J. (author), Smith, C.S. (author), Verbiest, G.J. (author), Katan, A.J. (author), Soloviev, O.A. (author), Verhaegen, M.H.G. (author)We propose to use the State Estimation by Sum-of-Norms Regularisation (STATESON-)algorithm for recovering the tip-sample interaction in high-speed tapping mode atomic force microscopy (AFM). This approach enables accurate sample height estimation for each independent cantilever oscillation period, provided that the tip-sample interaction...journal article 2023
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Fan, D. (author), Cnossen, J.P. (author), Hung, S. (author), Kromm, D. (author), Dekker, N.H. (author), Verbiest, G.J. (author), Smith, C.S. (author)To better understand the interactions between biological molecules, a high optical resolution in all three dimensions is crucial. The intrinsically lower axial resolution of microscopes however, is a limiting factor in fluorescence imaging, correspondingly in fluorescence based single molecule localization microscopy (SMLM). Here, we present...journal article 2023
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van der Werf, Verena M. (author), Zhao, J. (author), Koning, Jim S. (author), Nespoli, J. (author), Thieme, J. (author), Bus, M. (author), Savenije, T.J. (author)Metal-halide perovskites deposited by wet-chemical deposition have demonstrated great potential for various electronic applications, including solar cells. A remaining question is how light-induced excess charges become distributed over such polycrystalline material. Here, we examine the local conductive properties of MAPbI<sub>3</sub> and...journal article 2023
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Singh, Sukhvinder (author), Abelmann, L. (author), Gao, Haibin (author), Hartmann, Uwe (author)Well-defined and technically relevant domain configurations are sought in patterned magnetic thin films. We used Magnetic Force Microscopy to investigate these in square-patterned Permalloy films. The films were prepared using dc sputter deposition by varying the Argon pressure from 1.5×10−3 to 30.0×10−3 mbar. The Landau domain configuration was...journal article 2023
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Hebbar, Akshatha (author), Debraj, Devangshi (author), Acharya, Sriprasad (author), Puttapati, Sampath Kumar (author), Vatti, Anoop Kishore (author), Dey, P. (author)Deep eutectic solvents (DESs) are industrially promising solvents and posses numerous applications in wide areas such as metal processing, synthesis media, synthesis of nanoparticles, gas sequestration and many more. In this work, we synthesized and investigated the performance of three deep eutectic solvents (DESs) i.e., reline, glyceline...journal article 2023
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Wehrens, Martijn (author), Krah, Laurens H.J. (author), Towbin, Benjamin D. (author), Hermsen, Rutger (author), Tans, S.J. (author)The inherent stochasticity of metabolism raises a critical question for understanding homeostasis: are cellular processes regulated in response to internal fluctuations? Here, we show that, in E. coli cells under constant external conditions, catabolic enzyme expression continuously responds to metabolic fluctuations. The underlying...journal article 2023
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Tselentis, D. (author), Papadimitriou, E. (author)This study reviews the Artificial Intelligence and Machine Learning approaches developed thus far for driver profile and driving pattern recognition, representing a set of macroscopic and microscopic behaviors respectively, to enhance the understanding of human factors in road safety, and therefore reduce the number of crashes. It provides a...journal article 2023
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Abbassi-Daloii, Tooba (author), el Abdellaoui, Salma (author), Kan, H.E. (author), van den Akker, E.B. (author), ’t Hoen, Peter A.C. (author), Raz, Vered (author), Voortman, Lenard M. (author)Skeletal muscles are composed of different myofiber types characterized by the expression of myosin heavy chain isoforms, which can be affected by physical activity, aging, and pathological conditions. Here, we present a step-by-step high-throughput semi-automated approach for performing myofiber type quantification of entire human or mouse...journal article 2023
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Huang, R. (author), Zhao, Xuan (author), Yuan, Y. (author), Yu, Qiang (author), Liu, Chengqing (author), Daamen, W. (author)Pedestrian tactical choices and operational movement in evacuations essentially pertain to decision-making under risk and uncertainty. However, in microscopic evacuation models, this attribute has been greatly overlooked, even lacking a methodology to delineate the related decision characteristics (bounded rationality and risk attitudes), let...journal article 2023
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de Vries, J.J. (author), Laan, Daphne M. (author), Frey, F.F.F. (author), Koenderink, G.H. (author), de Maat, Moniek P.M. (author)Fibrous networks are essential structural components of biological and engineered materials. Accordingly, many approaches have been developed to quantify their structural properties, which define their material properties. However, a comprehensive overview and comparison of methods is lacking. Therefore, we systematically searched for...journal article 2023
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Voltage Imaging with Engineered Proton-Pumping Rhodopsins: Insights from the Proton Transfer PathwayMeng, X. (author), Ganapathy, S. (author), van Roemburg, Lars (author), Post, M.G. (author), Brinks, D. (author)Voltage imaging using genetically encoded voltage indicators (GEVIs) has taken the field of neuroscience by storm in the past decade. Its ability to create subcellular and network level readouts of electrical dynamics depends critically on the kinetics of the response to voltage of the indicator used. Engineered microbial rhodopsins form a...review 2023
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Ganapathy, S. (author), Meng, X. (author), Mossel, D.D.M. (author), Jagt, Mels (author), Brinks, D. (author)Genetically encoded voltage indicators, particularly those based on microbial rhodopsins, are gaining traction in neuroscience as fluorescent sensors for imaging voltage dynamics with high-spatiotemporal precision. Here we establish a novel genetically encoded voltage indicator candidate based on the recently discovered subfamily of the...journal article 2023
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Maddalena, L. (author), Pozzi, Paolo (author), Ceffa, Nicolò G. (author), Hoeven, Bas van der (author), Carroll, E.C.M. (author)Light-sheet microscopy is a powerful method for imaging small translucent samples in vivo, owing to its unique combination of fast imaging speeds, large field of view, and low phototoxicity. This chapter briefly reviews state-of-the-art technology for variations of light-sheet microscopy. We review recent examples of optogenetics in...book chapter 2023
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Martinez Torres, C.E. (author), Grimbergen, Jos (author), Koopman, Jaap (author), Koenderink, G.H. (author)Background: Fibrinogen is a plasma protein forming the fibrin scaffold of blood clots. Its mechanical properties therefore affect the risk of bleeding as well as thrombosis. There has been much recent interest in the biophysical mechanisms controlling fibrin mechanics; however, the role of molecular heterogeneity of the circulating fibrinogen...journal article 2023
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Veer, F.A. (author), Overend, M. (author), Sofokleous, E. (author), Noteboom, C. (author)Lack of knowledge about the properties of weathered (used) glass is currently a major barrier to glass reuse. This results in probably unnecessary recycling or down-cycling of architectural glass at the end of life. Avoiding this creates a significant opportunity to reduce resource depletion and decarbonize the built environment. This can be...journal article 2023