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Lakshminarayanan, Vasudevan (author), Chockalingam, Cindhuja (author), Mendes, E. (author), van Esch, J.H. (author)Properties such as shear modulus, gelation time, structure of supramolecular hydrogels are strongly dependent on self-assembly, gelation triggering mechanism and processes used to form the gel. In our work we extend reported rheology analysis methodologies to pH-triggered supramolecular gels to understand structural insight using a model...journal article 2021
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Storteboom, J. (author), Barbiero, Martina (author), Castelletto, Stefania (author), Gu, Min (author)The negatively charged nitrogen-vacancy (NV <sup>-</sup>) centre in nanodiamonds (NDs) has been recently studied for applications in cellular imaging due to its better photo-stability and biocompatibility if compared to other fluorophores. Super-resolution imaging achieving 20-nm resolution of NV <sup>-</sup> in NDs has been proved over the...journal article 2021
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Saeedifar, M. (author), Saghafi, Hamed (author), Mohammadi, Reza (author), Zarouchas, D. (author)The present study evaluates the toughening capability of electrospun PA66 nanofibers for carbon/epoxy composite laminates subjected to mode II loading conditions at elevated temperatures. The Dynamic Mechanical Analysis (DMA) test showed that the glass transition temperature of the produced nanofibers is in a range of ∼60–80 °C. Accordingly,...journal article 2021
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Xia, Fei (author), Gevers, Monique (author), Fognini, Andreas (author), Mok, Aaron T. (author), Li, Bo (author), Akbari, Najva (author), Esmaeil Zadeh, I.Z. (author), Qin-Dregely, Y. (author), Xu, Chris (author)Optical microscopy is a valuable tool for in vivo monitoring of biological structures and functions because of its noninvasiveness. However, imaging deep into biological tissues is challenging due to the scattering and absorption of light. Previous research has shown that the two optimal wavelength windows for high-resolution deep mouse brain...journal article 2021
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Neumann, Elizabeth K. (author), Patterson, Nathan Heath (author), Allen, Jamie L. (author), Migas, L.G. (author), Yang, Haichun (author), Brewer, Maya (author), Anderson, David M. (author), Harvey, Jennifer (author), Harris, Raymond C. (author), Van de Plas, Raf (author)Here, we describe the preservation and preparation of human kidney tissue for interrogation by histopathology, imaging mass spectrometry, and multiplexed immunofluorescence. Custom image registration and integration techniques are used to create cellular and molecular atlases of this organ system. Through careful optimization, we ensure high...journal article 2021
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Srinivasa Raja, A. (author), de Boer, Pascal (author), Giepmans, Ben N.G. (author), Hoogenboom, J.P. (author)Electron microscopy is crucial for imaging biological ultrastructure at nanometer resolution. However, electron irradiation also causes specimen damage, reflected in structural and chemical changes that can give rise to alternative signals. Here, luminescence induced by electron-beam irradiation is reported across a range of materials widely...journal article 2021
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Iv, Francois (author), Martins, Carla Silva (author), Castro Linares, G. (author), Taveneau, Cyntia (author), Barbier, Pascale (author), Verdier-Pinard, Pascal (author), Camoin, Luc (author), Audebert, Stéphane (author), Koenderink, G.H. (author)Septin GTP-binding proteins contribute essential biological functions that range from the establishment of cell polarity to animal tissue morphogenesis. Human septins in cells form hetero-octameric septin complexes containing the ubiquitously expressed SEPT9 subunit (also known as SEPTIN9). Despite the established role of SEPT9 in mammalian...journal article 2021
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Balasubramani, Vinoth (author), Kujawińska, Małgorzata (author), Allier, Cédric (author), Anand, Vijayakumar (author), Cheng, Chau Jern (author), Depeursinge, Christian (author), Hai, Nathaniel (author), Kalkman, J. (author), Park, Yong Keun (author)Quantitative Phase Imaging (QPI) provides unique means for the imaging of biological or technical microstructures, merging beneficial features identified with microscopy, interferometry, holography, and numerical computations. This roadmap article reviews several digital holography-based QPI approaches developed by prominent research groups....review 2021
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Legerstee, W.J. (author), Boekel, M.G.M. (author), Boonstra, S. (author), Kelder, E.M. (author)An Atomic Force Microscope (AFM) is combined with a special designed glovebox system and coupled to a Galvanostat/Potentiostat to allow measurements on electrochemical properties for battery research. An open cell design with electrical contacts makes it possible to reach the electrode surface with the cantilever so as to perform measurements...journal article 2021
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Van Der Burgt, Julia S. (author), Dieleman, Christian D. (author), Johlin, Eric (author), Geuchies, J.J. (author), Houtepen, A.J. (author), Ehrler, Bruno (author), Garnett, Erik C. (author)Accurately controlling light emission using nano- and microstructured lenses and antennas is an active field of research. Dielectrics are especially attractive lens materials due to their low optical losses over a broad bandwidth. In this work we measure highly directional light emission from patterned quantum dots (QDs) aligned underneath...journal article 2021
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Lane, R. (author), Vos, Y. (author), Wolters, A.E. (author), van Kessel, L.C.P.M. (author), Chen, S. Elisa (author), Liv, Nalan (author), Klumperman, Judith (author), Giepmans, Ben N.G. (author), Hoogenboom, J.P. (author)Large-scale electron microscopy (EM) allows analysis of both tissues and macromolecules in a semi-automated manner, but acquisition rate forms a bottleneck. We reasoned that a negative bias potential may be used to enhance signal collection, allowing shorter dwell times and thus increasing imaging speed. Negative bias potential has previously...journal article 2021
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Smith, Gregory N. (author), Brok, Erik (author), Schmiele, Martin (author), Mortensen, Kell (author), Bouwman, W.G. (author), Duif, C.P. (author), Hassenkam, Tue (author), Alm, Martin (author), Thomsen, Peter (author), Arleth, Lise (author)By introducing hydrophilic polymers into silicone medical devices, highly beneficial biomedical properties can be realized. An established solution to introduce hydrophilic polymers is to form an interpenetrating polymer network (IPN) by performing the hydrogel synthesis in the presence of silicone swollen in supercritical carbon dioxide. The...journal article 2021
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Kundarapu, Laxman Kumar (author), Choudhury, Satyajeet (author), Acharya, Sriprasad (author), Vatti, Anoop Kishore (author), Pandiyan, Sudharsan (author), Gadag, Shivaprasad (author), Nayak, Usha Yogendra (author), Dey, P. (author)The aggregation behavior of asphaltene in the toluene-hexane mixture is systematically investigated using experimental techniques such as optical microscopy and Fourier-transform infrared spectroscopy (FTIR) combined with molecular dynamics (MD) simulations. The optical images of various asphaltene concentrations are processed to determine...journal article 2021
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Navikas, Vytautas (author), Descloux, Adrien C. (author), Grußmayer, K.S. (author), Marion, Sanjin (author), Radenovic, Aleksandra (author)A variety of modern super-resolution microscopy methods provide researchers with previously inconceivable biological sample imaging opportunities at a molecular resolution. All of these techniques excel at imaging samples that are close to the coverslip, however imaging at large depths remains a challenge due to aberrations caused by the...journal article 2021
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Hingot, V. (author), Chavignon, A. (author), Heiles, B.G. (author), Couture, O. (author)The resolution of an imaging system is usually determined by the width of its point spread function and is measured using the Rayleigh criterion. For most system, it is in the order of the imaging wavelength. However, super resolution techniques such as localization microscopy in optical and ultrasound imaging can resolve features an order of...journal article 2021
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Santos, Luis F.P. (author), Ribeiro, Bruno (author), Hein, Luis R.O. (author), Alderliesten, R.C. (author), Zarouchas, D. (author), Botelho, Edson C. (author), Costa, Michelle L. (author)This work concerns the fatigue behavior at three different temperature conditions (−40, 20, and 80°C) and the addition of multiwalled carbon nanotube (MWCNT) into a carbon-fiber reinforced poly(ether-imide) composite. The incorporation of MWCNT into the composite increased the tensile strength and Young's modulus by up 5 and 2%, respectively....journal article 2020
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Janssen, V.A.E.C. (author)This thesis describes research into the interaction between electrons and various (pseudo) two-dimensional materials. This research is using two approaches: in Chapters 3 and 4 a low-energy electron microscope is used, and in Chapters 5 and 6 transport properties are studied. Chapter 1 introduces the concept of a two-dimensional material. First,...doctoral thesis 2020
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Büke, F. (author)Bacteria have been an integral part of human life since the ancient times either as cooperative tenants living in and around us or as constant threats to our health and wellbeing. Since prehistoric times we unknowingly used them as tools of fermentation and fought against them with haphazardly discovered natural remedies. Today, after more than...doctoral thesis 2020
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van Rooij, J. (author)Optical imaging is the imaging of objects with visible light. It is a tool often used for diagnostic purposes, such as in biomedical and material sciences. Digital holography is an optical imaging technique that captures and images the amplitude as well as the phase (the complex amplitude) of the lightwave. An advantage is that the complex...doctoral thesis 2020
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Deb Roy, S. (author)The basis of this thesis has been the curiosity, however modest, to understand how DNA replication happens in vivo, particularly during the onset of DNA damage and beyond. DNA damage is a recurring phenomenon, which a (bacterial) cell faces in its lifetime from the environment or even its inherent metabolism. While we understand much about...doctoral thesis 2020