Localization microscopy of constrained fluorescent molecules: Pushing towards Ångström-scale resolution through cryogenics

<i>Localization microscopy has circumvented the diffraction limit by sequentially</i> imaging individual light emitting molecules at a time. The position of these individual molecules can be determined and a super-resolution reconstruction is made with improved resolution. Normally freely rotating emitters are used such that the point spread...

Physical and Computational Approaches to Aberration Correction In Fluorescence Microscopy

The goal of this thesis, called Physical and Computational Approaches to Aberration Correction In Fluorescence Microscopy, concerns itself with the development of new techniques to control adaptive fluorescence microscopes, so that they can adapt and image with increased resolution, contrast and speed inside complex three-dimensional biological...

Photon-Counting Arrays for Time-Resolved Imaging

The paper presents a camera comprising 512 × 128 pixels capable of single-photon detection and gating with a maximum frame rate of 156 kfps. The photon capture is performed through a gated single-photon avalanche diode that generates a digital pulse upon photon detection and through a digital one-bit counter. Gray levels are obtained through...

Spatially periodic liquid crystal director field appearing in a photonic crystal template pectroscopy

Active tuning of photonic crystals can be achieved by filling the porous structures with liquid crystals. Here, the director field in macropores was studied by fluorescence confocal polarizing microscopy. For this purpose, the photonic crystal was infiltrated with a glass-forming liquid crystalline polymer, the sample was cooled below the glass...