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Legerstee, Karin (author), Sueters, J. (author), Abraham, Tsion E. (author), Slotman, Johan A. (author), Kremers, Gert Jan (author), Hoogenboom, J.P. (author), Houtsmuller, A.B. (author)Focal adhesions (FAs) are the main cellular structures to link the intracellular cytoskeleton to the extracellular matrix. FAs mediate cell adhesion, are important for cell migration and are involved in many (patho)-physiological processes. Here we examined FAs and their associated actin fibres using correlative fluorescence and scanning...journal article 2022
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Roobol, Stefan J. (author), Hartjes, Thomas A. (author), Slotman, Johan A. (author), de Kruijff, R.M. (author), Kanaar, R. (author), Houtsmuller, A.B. (author), Denkova, A.G. (author), van Royen, M.E. (author), Essers, J. (author)Polymersomes have the potential to be applied in targeted alpha radionuclide therapy, while in addition preventing release of recoiling daughter isotopes. In this study, we investigated the cellular uptake, post uptake processing and intracellular localization of polymersomes. Methods: High-content microscopy was used to validate polymersome...journal article 2020
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Yoo, H.W. (author), van Royen, M.E. (author), van Cappellen, WA (author), Houtsmuller, AB (author), Verhaegen, M.H.G. (author), Schitter, G (author)The pinhole plays an important role in confocal laser scanning microscopy (CLSM) for adaptive optics (AO) as well as in imaging, where the size of the pinhole denotes a trade-off between out-of-focus rejection and wavefront distortion. This contribution proposes an AO system for a commercial CLSM with an adjustable square pinhole to cope with...conference paper 2016