Optical tools towards the improvement of optogenetic stimulation

Optogenetics is a powerful addition to the spectrum of techniques available in neuroscience to investigate neurophysiology and unravel how neural circuit structure is related to circuit function. This technique relies on introducing lightsensitive proteins or molecules as actuators to transduce an optical signal into a physiological perturbation...

Adaptive optics in single objective inclined light sheet microscopy enables three-dimensional localization microscopy in adult Drosophila brains

Single-molecule localization microscopy (SMLM) enables the high-resolution visualization of organelle structures and the precise localization of individual proteins. However, the expected resolution is not achieved in tissue as the imaging conditions deteriorate. Sample-induced aberrations distort the point spread function (PSF), and high...

Adaptive optics enables multimode 3D super-resolution microscopy via remote focusing

A variety of modern super-resolution microscopy methods provide researchers with previously inconceivable biological sample imaging opportunities at a molecular resolution. All of these techniques excel at imaging samples that are close to the coverslip, however imaging at large depths remains a challenge due to aberrations caused by the...

Physical and Computational Approaches to Aberration Correction In Fluorescence Microscopy

The goal of this thesis, called Physical and Computational Approaches to Aberration Correction In Fluorescence Microscopy, concerns itself with the development of new techniques to control adaptive fluorescence microscopes, so that they can adapt and image with increased resolution, contrast and speed inside complex three-dimensional biological...