Searched for: subject%3A%22microscopy%22
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Iv, Francois (author), Martins, Carla Silva (author), Castro Linares, G. (author), Taveneau, Cyntia (author), Barbier, Pascale (author), Verdier-Pinard, Pascal (author), Camoin, Luc (author), Audebert, Stéphane (author), Koenderink, G.H. (author)
Septin GTP-binding proteins contribute essential biological functions that range from the establishment of cell polarity to animal tissue morphogenesis. Human septins in cells form hetero-octameric septin complexes containing the ubiquitously expressed SEPT9 subunit (also known as SEPTIN9). Despite the established role of SEPT9 in mammalian...
journal article 2021
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Quan, D. (author), Moloney, Peter (author), Carolan, Declan (author), Abourayana, Hisham (author), Ralph, Calvin (author), Ivankovic, Alojz (author), Dowling, Denis (author), Murphy, Neal (author)
This work studied the effects of adding short basalt fibers (BFs) and multi-walled carbon nanotubes (MWCNTs), both separately and in combination, on the mechanical properties, fracture toughness, and electrical conductivity of an epoxy polymer. The surfaces of the short BFs were either treated using a silane coupling agent or further...
journal article 2021
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Santos, Luis F.P. (author), Ribeiro, Bruno (author), Hein, Luis R.O. (author), Alderliesten, R.C. (author), Zarouchas, D. (author), Botelho, Edson C. (author), Costa, Michelle L. (author)
This work concerns the fatigue behavior at three different temperature conditions (−40, 20, and 80°C) and the addition of multiwalled carbon nanotube (MWCNT) into a carbon-fiber reinforced poly(ether-imide) composite. The incorporation of MWCNT into the composite increased the tensile strength and Young's modulus by up 5 and 2%, respectively....
journal article 2020
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Vasić, Borislav (author), Ralević, Uroš (author), Zobenica, Katarina Cvetanović (author), Smiljanić, Milče M. (author), Gajić, Radoš (author), Spasenović, Marko (author), Vollebregt, S. (author)
Chemical vapour deposition (CVD) is a promising method for producing large-scale graphene (Gr). Nevertheless, microscopic inhomogeneity of Gr grown on traditional metal substrates such as copper or nickel results in a spatial variation of Gr properties due to long wrinkles formed when the metal substrate shrinks during the cooling part of the...
journal article 2020
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van Kessel, L.C.P.M. (author), Huisman, T.A. (author), Hagen, Cornelis W. (author)
Background: Line-edge roughness (LER) is often measured from top-down critical dimension scanning electron microscope (CD-SEM) images. The true three-dimensional roughness profile of the sidewall is typically ignored in such analyses. Aim: We study the response of a CD-SEM to sidewall roughness (SWR) by simulation. Approach: We generate...
journal article 2020
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Vos, Y. (author), Lane, R. (author), Peddie, Chris J. (author), Wolters, A.E. (author), Hoogenboom, J.P. (author)
The authors present the application of a retarding field between the electron objective lens and sample in an integrated fluorescence and electron microscope. The retarding field enhances signal collection and signal strength in the electron microscope. This is beneficial for samples prepared for integrated fluorescence and electron...
journal article 2020
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Basak, Shibabrata (author), Baaij, Siemen (author), Ganapathy, S. (author), George, Chandramohan (author), Tempel, Hermann (author), Kungl, Hans (author), Kelder, E.M. (author), Zandbergen, H.W. (author), Wagemaker, M. (author), Eichel, Rüdiger A. (author)
High-fidelity and facile ex situ transmission electron microscopy (TEM) characterization of lithium−oxygen (Li−O<sub>2</sub>) batteries is still limited by challenges in preserving the native environment of Li−O<sub>2</sub> discharge products. The extreme reactivity and moisture sensitivity of the discharge products means that they are...
journal article 2020
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Mahgoub, A.M.I.M. (author), Lu, Hang (author), Thorman, Rachel M. (author), Preradovic, Konstantin (author), Jurca, Titel (author), McElwee-White, Lisa (author), Fairbrother, Howard (author), Hagen, C.W. (author)
Two platinum precursors, Pt(CO)<sub>2</sub>Cl<sub>2</sub> and Pt(CO)<sub>2</sub>Br<sub>2</sub>, were designed for focused electron beam-induced deposition (FEBID) with the aim of producing platinum deposits of higher purity than those deposited from commercially available precursors. In this work, we present the first deposition experiments...
journal article 2020
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Wong, H.S. (author), Poole, A.B. (author), Wells, B. (author), Eden, M. (author), Barnes, R. (author), Ferrari, J. (author), Fox, R. (author), Yio, M.H.N. (author), Copuroglu, Oguzhan (author)
Water to cement (w/c) ratio is usually the most important parameter specified in concrete design and is sometimes the subject of dispute when a shortfall in concrete strength or durability is an issue. However, determination of w/c ratio in hardened concrete by testing is very difficult once the concrete has set. This paper presents the...
journal article 2020
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Filius, M. (author), Cui, T.J. (author), Ananth, A.N. (author), Docter, M.W. (author), Hegge, Jorrit W. (author), van der Oost, John (author), Joo, C. (author)
Super-resolution imaging allows for the visualization of cellular structures on a nanoscale level. DNA-PAINT (DNA point accumulation in nanoscale topology) is a super-resolution method that depends on the binding and unbinding of DNA imager strands. The current DNA-PAINT technique suffers from slow acquisition due to the low binding rate of...
journal article 2020
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Zhang, Xukai (author), Ioannidou, C. (author), ten Brink, Gert H. (author), Navarro Lopez, A. (author), Wormann, Jan (author), Campaniello, Jean (author), Dalgliesh, Robert M. (author), van Well, A.A. (author), Offerman, S.E. (author), Kranendonk, Winfried (author), Kooi, Bart J. (author)
A cold-rolled Ti-V high strength low alloy (HSLA) steel was isothermally annealed at 650 °C and 700 °C for different times. A unique combination of techniques including visible light microscopy (VLM), transmission electron microscopy (TEM), matrix dissolution, small angle neutron scattering (SANS) and hardness measurement has been employed to...
journal article 2020
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Cichocka, M.O. (author), Bolhuis, M. (author), van Heijst, S.E. (author), Conesa Boj, S. (author)
Layered materials (LMs) such as graphene or MoS2 have attracted a great deal of interest recently. These materials offer unique functionalities due to their structural anisotropy characterized by weak van der Waals bonds along the out-of-plane axis and covalent bonds in the in-plane direction. A central requirement to access the structural...
journal article 2020
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Fu, J. (author), van Slingerland, J. (author), Brouwer, J.C. (author), Bliznuk, Vitaliy (author), Richardson, I.M. (author), Hermans, M.J.M. (author)
Pulsed laser beam welding was used successfully to join the oxide dispersion-strengthened (ODS) Eurofer steel. The joining was conducted with a laser power of 2500 W and a pulsed duration of 4 ms. With the filler material being used, a minor material loss and microvoids were observed in the joint. The microstructure of the fusion zone...
journal article 2020
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Huisman, D. J. (author), Bach, A. (author), Ngan-Tillard, D.J.M. (author), Joosten, I. (author), van den Eynde, G. (author)
We investigated the characteristics of a group of 13 Middle Iron Age egg-shaped crucibles and crucible fragments from Tilburg (The Netherlands). We used a combination of optical and chemical analyses, including hand-held XRF, microCT scanning and 3-D printing polarizing light microscopy and SEM-EDX. The chemical analyses confirmed that the...
journal article 2020
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Basak, S. (author), Migunov, Vadim (author), Lee, Q. (author), Ganapathy, S. (author), Vijay, Ashwin (author), Ooms, F.G.B. (author), Wagemaker, M. (author), Kelder, E.M. (author), Arszelewska, Violetta (author)
With operando transmission electron microscopy visualizing the solid-solid electrode-electrolyte interface of silicon active particles and lithium oxide solid electrolyte as a model system, we show that (de)lithiation (battery cycling) does not require all particles to be in direct contact with electrolytes across length scales of a few...
journal article 2020
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Beekers, D.I. (author), Mastik, Frits (author), Beurskens, Robert (author), Tang, Phoei Ying (author), Vegter, Merel (author), van der Steen, A.F.W. (author), de Jong, N. (author), Verweij, M.D. (author), Kooiman, Klazina (author)
Ultrasound insonification of microbubbles can locally enhance drug delivery, but the microbubble–cell interaction remains poorly understood. Because intracellular calcium (Ca<sub>i</sub> <sup>2+</sup>) is a key cellular regulator, unraveling the Ca<sub>i</sub> <sup>2+</sup> fluctuations caused by an oscillating microbubble provides crucial...
journal article 2020
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Roobol, Stefan J. (author), Hartjes, Thomas A. (author), Slotman, Johan A. (author), de Kruijff, R.M. (author), Kanaar, R. (author), Houtsmuller, A.B. (author), Denkova, A.G. (author), van Royen, M.E. (author), Essers, J. (author)
Polymersomes have the potential to be applied in targeted alpha radionuclide therapy, while in addition preventing release of recoiling daughter isotopes. In this study, we investigated the cellular uptake, post uptake processing and intracellular localization of polymersomes. Methods: High-content microscopy was used to validate polymersome...
journal article 2020
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Zhang, L. (author), Garming, M.W.H. (author), Hoogenboom, J.P. (author), Kruit, P. (author)
Electrostatic beam blankers are an alternative to photo-emission sources for generating pulsed electron beams for Time-resolved Cathodoluminescence and Ultrafast Electron Microscopy. While the properties of beam blankers have been extensively investigated in the past for applications in lithography, characteristics such as the influence of...
journal article 2020
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Vigueras Guillén, J.P. (author), Sari, B. (author), Goes, S.F. (author), Lemij, Hans G. (author), van Rooij, Jeroen (author), Vermeer, K.A. (author), van Vliet, L.J. (author)
Background<br/><br/>Corneal endothelium (CE) images provide valuable clinical information regarding the health state of the cornea. Computation of the clinical morphometric parameters requires the segmentation of endothelial cell images. Current techniques to image the endothelium in vivo deliver low quality images, which makes automatic...
journal article 2019
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Pardanaud, Cedric (author), Cartry, Gilles (author), Lajaunie, Luc (author), Arenal, Raul (author), Buijnsters, J.G. (author)
Multiwavelength Raman spectroscopy (325, 514, 633 nm) was used to analyze three different kinds of samples containing sp2 and sp3 carbons: chemical vapor deposited diamond films of varying microstructure, a plasma-enhanced chemical vapor deposited hydrogenated amorphous carbon film heated at 500 °C and highly oriented pyrolytic graphite exposed...
journal article 2019
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