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Solis Escalante, D. (author)Glycolysis, a biochemical pathway that oxidizes glucose to pyruvate, is at the core of sugar metabolism in Saccharomyces cerevisiae (bakers’ yeast). Glycolysis is not only a catabolic route involved in energy conservation, but also provides building blocks for anabolism. From an applied perspective, several glycolytic intermediates are key...doctoral thesis 2015
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Ederveen, A. (author), Lai, Y. (author), Van Driel, M.A. (author), Gerats, T. (author), Peters, J.L. (author)Background Crossing over assures the correct segregation of the homologous chromosomes to both poles of the dividing meiocyte. This exchange of DNA creates new allelic combinations thus increasing the genetic variation present in offspring. Crossovers are not uniformly distributed along chromosomes; rather there are preferred locations where...journal article 2015
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Tiruvadi Krishnan, S. (author), Moolman, M.C. (author), van Laar, T. (author), Meyer, A.S. (author), Dekker, N.H. (author)Background: Chromosome engineering encompasses a collection of homologous recombination-based techniques that are employed to modify the genome of a model organism in a controlled fashion. Such techniques are widely used in both fundamental and industrial research to introduce multiple insertions in the same Escherichia coli strain. To date,...journal article 2015
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Jorda, J. (author), Cueto Rojas, H.F. (author), Carnicer, M. (author), Wahl, S.A. (author), Ferrer, P. (author), Albiol, J. (author)Pichia pastoris has been recognized as an effective host for recombinant protein production. In this work, we combine metabolomics and instationary 13C metabolic flux analysis (INST 13C-MFA) using GC-MS and LC-MS/MS to evaluate the potential impact of the production of a Rhizopus oryzae lipase (Rol) on P. pastoris central carbon metabolism....journal article 2014
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Kuijpers, N.G.A. (author), Chroumpi, S. (author), Vos, T. (author), Solis-Escalante, D. (author), Bosman, D. (author), Pronk, J.T. (author), Daran, J.G. (author), Daran-Lapujade, P.A.S. (author)In vivo assembly of overlapping fragments by homologous recombination in Saccharomyces cerevisiae is a powerful method to engineer large DNA constructs. Whereas most in vivo assembly methods reported to date result in circular vectors, stable integrated constructs are often preferred for metabolic engineering as they are required for large-scale...journal article 2013
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Van Loenhout, M.T.J. (author)This thesis describes a series of single-molecule experiments aimed at understanding the physical properties of DNA itself and the proteins that interact with it. We developed and applied sensitive techniques that allowed us to directly probe the conformation and interactions of individual DNA molecules and proteins. Magnetic and optical...doctoral thesis 2012
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Carnicer, M. (author), Ten Pierick, A. (author), Van Dam, J. (author), Heijnen, J.J. (author), Albiol, J. (author), Van Gulik, W. (author), Ferrer, P. (author)Background: Environmental and intrinsic stress factors can result in the global alteration of yeast physiology, as evidenced by several transcriptional studies. Hypoxia has been shown to have a beneficial effect on the expression of recombinant proteins in Pichia pastoris growing on glucose. Furthermore, transcriptional profiling analyses...journal article 2012
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Louwers, D.J. (author), Takizawa, T. (author), Hidaka, C. (author), Van der Kolk, E. (author)A Eu2+ concentration and temperature dependent energy transfer study from the host lattice to Eu2+ luminescence centers in Ca(1–x)EuxGa2S4 (x = 0.001 to 0.05) was performed with a special streak camera that combines the high timing resolution of a conventional synchroscan operation (<2 ps) with the ability to study long lived states (10?ns???1...journal article 2012
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De Visser, P.J. (author), Baselmans, J.J.A. (author), Diener, P. (author), Yates, S.J.C. (author), Endo, A. (author), Klapwijk, T.M. (author)We present measurements of quasiparticle generation-recombination noise in aluminium Microwave Kinetic Inductance Detectors, the fundamental noise source for these detectors. Both the quasiparticle lifetime and the number of quasiparticles can be determined from the noise spectra. The number of quasiparticles saturates to 10 ?m?3 at temperatures...journal article 2012
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Kind, R. (author), Van Swaaij, R.A.C.M.M. (author), Rubinelli, F.A. (author), Solntsev, S. (author), Zeman, M. (author)The performance of hydrogenated amorphous silicon (a-Si:H) p-i-n solar cells is limited, as they contain a relatively high concentration of defects. The dark current voltage (JV) characteristics at low forward voltages of these devices are dominated by recombination processes. The recombination rate depends on the concentration of active...journal article 2011
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Illiberi, A. (author), Kudlacek, P. (author), Smets, A.H.M. (author), Creatore, M. (author), Van de Sanden, M.C.M. (author)We have found that controlled Ar ion bombardment enhances the degradation of a-Si:H based surface passivation of c-Si surfaces. The decrease in the level of surface passivation is found to be independent on the ion kinetic energy (7–70 eV), but linearly proportional to the ion flux (6×1014–6×1015?ions?cm?2?s?1). This result suggests that the ion...journal article 2011
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Hiseni, A. (author), Arends, I.W.C.E. (author), Otten, L.G. (author)Two carotenoid 1,2-hydratase (CrtC) genes from the photosynthetic bacteria Rubrivivax gelatinosus and Thiocapsa roseopersicina were cloned and expressed in Escherichia coli in an active form and purified by affinity chromatography. The biochemical properties of the recombinant enzymes and their substrate specificities were studied. The purified...journal article 2011
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- Hofhuis, J.P.T. (author) doctoral thesis 2009
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Barends, R. (author)One of the greatest challenges in astronomy is observing star and planetary formation, redshifted distant galaxies and molecular spectral ‘fingerprints’ in the far-infrared spectrum of light, using highly sensitive and large cameras. In this thesis we investigate superconducting resonators for photon detection. In superconductors the electrons...doctoral thesis 2009
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van der Heijden, A.H. (author)What happens when your DNA breaks? This thesis describes experimental work on the single-molecule level focusing on the interaction between DNA and DNA-repair proteins, in particular bacterial RecA and human Rad51, involved in homologous recombination. Homologous recombination and its central event of DNA strand exchange are essential processes...doctoral thesis 2007
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Yan, F. (author), Migliorato, P. (author), Ishihara, R. (author)The influence of twin boundaries on the characteristics of single grain-silicon thin film transistors has been analyzed by three-dimensional simulation. The simulations show that the orientation and the location of a twin boundary could affect the field-effect mobility and the leakage current of a device. The field-effect mobility increases with...journal article 2007
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Savenije, T.J. (author), Nanu, M. (author), Schoonman, J. (author), Goossens, A. (author)Photoinduced interfacial charge carrier generation, separation, trapping, and recombination in TiO2?In2S3?CuInS2 cells have been studied with time-resolved microwave conductivity (TRMC). Single layer, double layer, and complete triple layer configurations have been studied. Selective electronic excitation in one of the components is accomplished...journal article 2007
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- Dekker, C. (author), Scholten, A.J. (author), Liefrink, F. (author), Eppenga, R. (author), Van Houten, H. (author), Foxon, C.T. (author) journal article 1991
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- Goossens, A. (author), Schoonman, J. (author) journal article 1990
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