Single-Molecule FRET X

Fluorescence resonance energy transfer (FRET) is a photophysical phenomenon that has been repurposed as a biophysical tool to measure nanometer distances. With FRET by DNA eXchange, or FRET X, many points of interest (POIs) in a single object can be probed, overcoming a major limitation of conventional single-molecule FRET. In FRET X, short...

High-Resolution Single-Molecule FRET via DNA eXchange (FRET X)

Single-molecule FRET is a versatile tool to study nucleic acids and proteins at the nanometer scale. However, currently, only a couple of FRET pairs can be reliably measured on a single object, which makes it difficult to apply single-molecule FRET for structural analysis of biomolecules. Here, we present an approach that allows for the...

Seek and Destroy: Single-molecule perspective on the target search and recognition by the Cas9 endonuclease

The most commonly known relationship between the macromolecules of life – DNA, RNA and protein – is the central dogma which states that information flows from DNA to RNA through transcription and from RNA to protein through translation. The discovery of non-coding RNAs has altered and expanded this picture by revealing many more functions of RNA...