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McKenzie, R. (author), Keizer, Emma M. (author), Vink, J.N.A. (author), van Lopik, Jasper (author), Büke, F. (author), Kalkman, Vera (author), Fleck, Christian (author), Tans, S.J. (author), Brouns, S.J.J. (author)While CRISPR-Cas defence mechanisms have been studied on a population level, their temporal dynamics and variability in individual cells have remained unknown. Using a microfluidic device, time-lapse microscopy and mathematical modelling, we studied invader clearance in Escherichia coli across multiple generations. We observed that CRISPR...journal article 2022
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Kieper, S.N. (author), Almendros Romero, C. (author), Behler, Juliane (author), McKenzie, R. (author), Luzia de Nobrega, F. (author), van Eijkeren-Haagsma, A.C. (author), Vink, J.N.A. (author), Hess, Wolfgang R. (author), Brouns, S.J.J. (author)CRISPR-Cas systems adapt their immunological memory against their invaders by integrating short DNA fragments into clustered regularly interspaced short palindromic repeat (CRISPR) loci. While Cas1 and Cas2 make up the core machinery of the CRISPR integration process, various class I and II CRISPR-Cas systems encode Cas4 proteins for which...journal article 2018