This thesis presents the development of an open-source electronic design automation (EDA) toolchain for mixed-signal integrated circuit (IC) design using the Skywater 130nm PDK. The toolchain integrates digital, analog, and mixed-signal flows using OpenLane, Xschem, Ngspice, and ALIGN, among others. It automates key steps such as schematic simulation, layout generation, and verification while remaining accessible and modular. A major contribution is the demonstration of cryogenic simulation support using extracted parameters from transistor measurements at 4K, showcasing the potential for space and quantum applications. The work also includes Docker-based deployment to ensure reproducibility and ease of use across platforms.