Cosmic ray neutron sensor (CRNS) has gained popularity in the last decade for its suitability in estimating area-averaged soil moisture (SM). The presence of fresh biomass influences the CRNS signal due to its water content, introducing bias to soil moisture estimation. Calibration and correction methods have been developed to account for this bias, but they usually require laborious sampling. Here, a novel approach is tested to assess the impact of biomass water equivalent (BWE) on CRNS soil moisture estimation. It was conducted in two contrasting environments from 15/11/21–1/02/23 for an olive orchard in Saudi Arabia, and from 15/02/22–30/03/23 for a cherry orchard in France. Water-uptake rates were monitored using sap flow sensors, as well as actual evapotranspiration (AET) and in-situ SM within the CRNS footprint. Concurrent environmental variables were also measured with a research-grade weather stations. It was found that when vapor pressure deficit (VPD) > 1.8kPa, CRNS-derived SM (CRNS-SM) closely matched in-situ SM measurements, which indicates minimal influence from BWE. Conversely, when VPD is lower than 1.8kPa, CRNS-SM overestimates the in-situ moisture. An optimization approach was used to find a temporally-varying value of N₀ parameter that minimizes the difference between soil moisture estimated with CRNS and in-situ sensors. Furthermore, the results showed that the relative change in the optimized value of N₀ (N_0,opt) was well correlated with VPD in both orchards (R² = 0.66 for olive and R² = 0.74 for cherry orchards), indicating a strong correlation between these variables. These findings suggest that integrating VPD and CRNS observations, and using the VPD-N_0,opt correlation approach could be a promising way to account for the bias due to biomass dynamics on the estimation of area-averaged SM.