Daan J.W. Brocken

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Journal article (1)

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Direct Visualization of Native CRISPR Target Search in Live Bacteria Reveals Cascade DNA Surveillance Mechanism

Journal article (2020) - Jochem N.A. Vink , Koen J.A. Martens , Marnix Vlot , Rebecca E. McKenzie , Cristóbal Almendros , Boris Estrada Bonilla , Daan J.W. Brocken , Johannes Hohlbein , Stan J.J. Brouns

Vink et al. tracked single CRISPR RNA-surveillance complexes (Cascade) in the native host cell and determined the influence of Cascade copy numbers, PAM scanning speed, and the presence of CRISPR arrays and transcription on their ability to find and clear invading mobile genetic ...