Direct Visualization of Native CRISPR Target Search in Live Bacteria Reveals Cascade DNA Surveillance Mechanism

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Direct Visualization of Native CRISPR Target Search in Live Bacteria Reveals Cascade DNA Surveillance Mechanism

Journal Article (2020)

Author(s)

Jochem N.A. Vink (Kavli institute of nanoscience Delft, TU Delft - Applied Sciences)

Koen J.A. Martens (Wageningen University & Research)

Marnix Vlot (Wageningen University & Research)

Rebecca E. McKenzie (Kavli institute of nanoscience Delft, TU Delft - Applied Sciences)

Cristóbal Almendros (Kavli institute of nanoscience Delft, TU Delft - Applied Sciences)

Boris Estrada Bonilla (TU Delft - Applied Sciences, Kavli institute of nanoscience Delft)

Daan J.W. Brocken (Gorlaeus Laboratories)

Johannes Hohlbein (Wageningen University & Research)

Stan J.J. Brouns (Kavli institute of nanoscience Delft, TU Delft - Applied Sciences)

Research Group

BN/Stan Brouns Lab

CRISPR-Cas PALM Single molecule Target search Single-particle tracking PAM Cascade Arms race Photo-activation localization microscopy

DOI related publication

https://doi.org/10.1016/j.molcel.2019.10.021 Final published version

To reference this document use

https://resolver.tudelft.nl/uuid:632388bb-9d42-4863-b88c-88e97e3255bf

More Info

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Publication Year

2020

Language

English

Research Group

BN/Stan Brouns Lab

Journal title

Molecular Cell

Issue number

1

Volume number

77

Pages (from-to)

39-50.e10

Downloads counter

350

Abstract

Vink et al. tracked single CRISPR RNA-surveillance complexes (Cascade) in the native host cell and determined the influence of Cascade copy numbers, PAM scanning speed, and the presence of CRISPR arrays and transcription on their ability to find and clear invading mobile genetic elements from the cell.