Abstract - Background Most widely used musculoskeletal models are predominantly based on male anatomy. This limits accurate biomechanical analysis in women, despite notable sex differences in occurrence of musculoskeletal pathologies. Aim This study aimed to develop a female musculoskeletal model of the lower extremity (YONI). The YONI is compared to the generic (male-based) model in simulation with a female participant. Methods The YONI model was developed in OpenSim Creator based on MR images. Comparisons were done between the YONI model and a scaled RAJAG model and a personalized model of using motion capture data in simulations. Results For comparison between YONI and RAJAG with another female subject, mean RMS error for the personalized model was 0.0096 m (SD = 0.0013), for the scaled RAJAG model 0.0247 m (SD = 0.0268) and for the scaled YONI model 0.0097 m (SD = 0.0010). Although SPM paired t-test showed significant differences for both YONI and RAJAG compared to the personalized model for all joint angles, the YONI model showed lower t-values compared to RAJAG. Joint moments reveal larger differences between YONI and scaled RAJAG models in the hip angles during the swing phase. Reserve moments were low in hip flexion and hip adduction, but higher in knee flexion and ankle flexion. Conclusion While observed kinematic and dynamic differences require cautious interpretation due to model limitations and data constraints, this work represents a crucial step toward the development of a female musculoskeletal model, essential for advancing biomechanical research and clinical applications for women.