This study aims to investigate the consumer acceptance of Vehicle-to-Grid (V2G) charging of electric vehicle (EV) drivers. To the best of the authors’ knowledge, this is the first V2G acceptance study that is based on actual users’ experience of V2G charging. A test set up with a V2G charge point at a solar carport was constructed at the Delft University of Technology. Seventeen participants in the study were given access to a V2G-compatible Nissan LEAF and the constructed V2G charging facilities, after which they were interviewed. Clear communication of the impacts of V2G charging cycles on EV batteries, financial compensation covering these impacts, real-time insight on the battery state-of-charge and the ability to set operational parameters through a user-friendly interface were all found to foster acceptance. The main barriers for acceptance were the uncertainty associated with battery state-of-charge, the increased need for planning charging and trips, the increased anxiety about the ability of the vehicle to reach its destination, economic and performance-related effects on the EV’s battery and the restriction of the freedom that users associated with their personal vehicles. The participants were found to be divided across high, conditional and low acceptance of V2G charging. The use of V2G charging over the trial period was found to inform their opinions: tangible factors such as range anxiety and the user interface were given more importance than abstract concepts such as lack of standards that were discussed by users without experience of V2G charging. Our study indicates that V2G charging in its current form is acceptable to a section of current EV users. The discussion provides insights on extending the relevance of our findings across other user groups and over further developments in the field.