This paper presents the results for an experimental campaign of in-situ impact during tension-tension fatigue loading for open-hole carbon fibre reinforced polymer specimens. High-speed low energy impact was introduced to the specimen with the use of a canon, which was attached to testing bench enabling the impact without the need to remove the specimens from the test bench. Digital Image Correlation, C-scan and Acoustic Emission were utilized to record health monitoring data for damage diagnostics. A strain-based criterion was used to identify a common threshold for the timing of impact ensuring a fair comparison between the different tests. The results indicate that while an impact causes the total amount of damage to increase as one would expect, it does not necessarily increase the damage level in the critical area where final fracture occurs. A dependence on the moment of impact with the fatigue failure was found for specimens subjected to impact before the initiation of the fatigue loading. In contrast, impacting specimens in the presence of fatigue damage had no detrimental effect on the fatigue life, although it was observed that the damaged area was enlarged. Overall, the paper showcases the need to study systemically the effect of in-situ impact on the fatigue life in order to understand better the implications that may be introduced to the integrity of a composite structure.