Co-fermentation of mixed sugars to produce butanol is an attractive route in sucrochemical production chains. Herein, high-level mixed sugars from sugarcane bagasse hemicellulosic hydrolysate (HH) and molasses (SCM) were investigated as potential substrates for acetone-butanol-ethanol (ABE) production in batch fermentation by Clostridium saccharoperbutylacetonicum DSM 14923. HH produced after hydrothermal pretreatment was concentrated 5-fold and was called concentrated hemicellulosic hydrolysate (CHH). The fermentation media that were investigated consisted solely of CHH and SCM and three CHH-to-SCM ratios were used to provide 30 g/L initial sugar concentrations and furan derivatives lower than 0.1 g/L. For CHH50/SCM50 and CHH75/SCM25, diluting CHH to concentrations of 15 g/L sugars and 22.5 g/L sugars, respectively, which were supplemented with SCM and nutrients, suffered growth inhibition as a function of the concentration of undissociated acid in the medium. The best-performing medium, CHH25/SCM75 (7.5 g/L and 22.5 g/L sugars from CHH and SCM, respectively and about 0.017 g/L furan derivatives), showed 97 % sugar consumption, and in the pH range of 5.5–6.5, undissociated acetic acid was not an inhibitory molecular form to C. saccharoperbutylacetonicum. After 30 h of fermentation, 7.8 and 9.8 g/L butanol and ABE were produced, respectively, which yielded 0.28 and 0.36 g/g. Based on our findings, C. saccharoperbutylacetonicum exhibits its potential and effective application for renewable butanol production by co-fermenting mixed sugars.@en

Robust strains are essential towards success of n-butanol production from lignocellulosic feedstock. To find a suitable strain to convert a non-detoxified hemicellulosic hydrolysate of sugarcane bagasse, we first assessed the performance of four wild-type butanol-producing Clostridium strains (C. acetobutylicum DSM 6228, C. beijerinckii DSM 6422, C. saccharobutylicum DSM 13864, and C. saccharoperbutylacetonicum DSM 14923)in batch fermentations containing either xylose or glucose at 30 g L−1 as sole carbon sources. C. saccharoperbutylacetonicum was selected after achieving butanol yields as high as 0.31 g g−1 on glucose and 0.25 g g−1 on xylose. In a 48-h fermentation containing a mixture of sugars (93% xylose and 7% glucose)that mimicked the hydrolysate, C. saccharoperbutylacetonicum delivered the highest butanol concentration (14.5 g L−1)when the initial sugar concentration was 50 g L−1. Moreover, the selected strain achieved the highest butanol yield (0.29 g g−1)on xylose-rich media reported so far. Meanwhile, C. saccharoperbutylacetonicum produced 5.8 g butanol L−1 (0.22 g g−1 butanol yield)when fermenting a non-detoxified sugarcane bagasse hemicellulosic hydrolysate enriched with xylose (30 g total sugars L−1). Although sugars were not exhausted (4.7 g residual sugars L−1)even after 72 h because of the presence of lignocellulose-derived microbial inhibitors, these results show that C. saccharoperbutylacetonicum is a robust wild-type strain. This microorganism with high butanol tolerance and yield on xylose can, therefore, serve as the basis for the development of improved biocatalysts for production of butanol from non-detoxified sugarcane bagasse hemicellulosic hydrolysate.@en

The aeronautical sector faces challenges in meeting its net-zero ambition by 2050. To achieve this target, much effort has been devoted to exploring sustainable aviation fuels (SAF). Accordingly, we evaluated the technical performance of potential SAF production in an integrated first- and second-generation sugarcane biorefinery focusing on Brazil. The CO2 equivalent and the renewability exergy indexes were used to assess environmental performance and impact throughout the supply chain. In addition, exergy efficiency (ηB) and average unitary exergy costs (AUEC) were used as complementary metrics to carry out a multi-criteria approach to determine the overall performance of the biorefinery pathways. The production capacity assumed for this analysis covers 10% of the fuel demand in 2020 at the international Brazilian airports of São Paulo and Rio de Janeiro, leading to a base capacity of 210 kt jet fuel/y. The process design includes sugarcane bagasse and straw as the feedstock of the biochemical processes, including diverse pre-treatment methods to convert lignocellulosic resources to biojet fuel, and lignin upgrade alternatives (cogeneration, fast pyrolysis, and gasification Fischer-Tropsch). The environmental analysis for all scenarios shows a GHG reduction potential due to a decrease of up to 30% in the CO2 equivalent exergy base emissions compared to fossil-based jet fuel.@en