Implant-associated infections remain a major challenge in orthopaedic surgery, motivating the development of antimicrobial implant surfaces that do not compromise bone regeneration. As osteoclasts are crucial for bone remodelling and implant integration, their response to antimi- crobial surface modifications must be carefully evaluated. This thesis investigated the effects of silver nanoparticles (AgNPs) incorporated into plasma electrolytic oxidation (PEO) treated Ti6Al4V implant surfaces on osteoclastogenesis under direct and indirect in vitro exposure con- ditions. Human CD14+ monocytes were cultured directly on the implant surfaces to evaluate surface-mediated effects, and indirectly in Transwell-based mono- and coculture models to as- sess the influence of released ions and human mesenchymal stem cell (hMSC)-derived signals on osteoclastogenesis. Surface characterisation confirmed successful PEO treatment and AgNP incorporation without alteration of surface morphology. Direct contact with AgNP-containing surfaces reduced the number of viable adherent cells, while osteoclast differentiation markers re- mained detectable in surviving cells. In contrast, indirect exposure to implant-derived ionic cues did not impair osteoclast formation. These findings highlight the exposure-dependent effects of AgNP-incorporated PEO surfaces and support their potential for antimicrobial implant design within a biologically tolerable range.