Welding is a joining method for thermoplastic composites (TPCs) that offers multiple advantages over the more traditional methods of mechanical fastening and adhesive bonding. A particularly promising welding technique is ultrasonic welding, which features very short process times as a result of the high heating rates that can be achieved. This spot welding technique is hypothesized to have potential for improved damage tolerance compared to more commonly used continuous welding techniques: in a multi-spot welded joint, evolving damage will need to re-initiate in subsequent spots. The fact that damage initiation will need to occur multiple times might delay overall damage evolution through the joint compared to a continuous welded joint, where damage initiation needs to occur only once. This work is a first exploratory step into the domain of fatigue of multi-spot welded joints in TPCs. Existing research on the fatigue behavior of four-spot welded steel joints in various layouts served as the main reference throughout this research: its methodology was transferred to four-spot welded joints in TPCs. By comparing fatigue behavior across both materials, it was evaluated to what extent existing knowledge and design rules for steel could potentially be transferred to TPCs. Differences were observed in the results obtained for TPC and steel joints. Most notably, in steel joints the dominant failure mode was seen to change from spot fracture to sheet fracture at higher fatigue lives. In TPCs, joints consistently showed spot fracture across all load levels. A different interrelation between layout performances was seen in the steel and thermoplastic composite joints, assumed to be a result of localized material strengthening in the steel joints from interference of adjacent heat-affected zones. These results indicate that existing knowledge on multi-spot welded joints in steel cannot be readily transferred to TPCs, as failure modes and material mechanisms may differ. It was discovered that, when one spot failed prematurely as a result of existing damage in the joint, the remaining layout no longer seemed to have an effect on fatigue life performance. This was attributed to asymmetry in the remaining joint layout, meaning one spot would always become a preferred location for damage initiation and subsequent evolution. Therefore, subsequent damage evolution would only be restricted by a single spot up to the point where the shear strength of the joint was exceeded.