A major challenge associated with the implementation of city logistics (CL) initiatives lies with their economic and financial long‐term success. The business model concept can support assessing the business side of stakeholders' decision‐making processes as major determinants for such success. A conceptual model is built from a role‐based business ecosystem modeling approach to provide a business model representation of the CL business ecosystem able to identify and explore the components of the system and their dynamics. The chapter presents the CL ecosystem business model framework and depicts some CL concepts. The CL role‐based business model framework follows the business ecosystem analysis and modeling (BEAM) approach. The framework is built for defining and structuring a wide range of business model configurations of roles and business entities in a CL system. The chapter then shows a process for the formalization required for the Agent‐Based Model implementation. @en