This paper investigates surfactant flooding using a microfluidic device. Its purpose is to validate the results obtained in recent core experiments reported by Janssen et al. (2019) and provide mechanistic interpretation. In these experiments authors injected a surfactant slug into sandstone cores, initially brought to residual oil saturation. Low oil/water IFT leads to oil mobilization and the formation of emulsions. Furthermore, authors found that oil is more effectively mobilized when the injected surfactant is at optimum salinity. In this study the following experiments are subsequently conducted in a microfluidic device to validate this optimum surfactant slug: primary drainage, waterflooding and surfactant flooding. In case of the surfactant flooding experiment, the micro-emulsion formation was observed at 1.5 PV and 1.25 PV for the under-optimum and optimum conditions respectively. In both conditions, an increased oil mobilization was obtained, compared to the waterflooding experiment. However, the optimum condition, with a slug salinity of 2.0 wt% NaCl outperformed the under-optimum condition with a slug salinity of 0.4 wt% NaCl in terms of oil recovery. The increased salinity in case of the optimum condition results in an lower IFT compared to the under-optimum condition, which in turn results in an higher capillary number. The obtained results indicate a dependency between the capillary number and the amount of oil droplets. The optimum capillary number can be found at the lowest oil saturation with the largest amount of droplets. Based on the results obtained from the conducted experiments on this microfluidic device, upscaling is considered to be a viable and educated recommendation.