SH
Sander Den Hoedt
Authored
1 records found
Cryogenic electron tomography (cryo-ET) combined with sub-tomogram averaging, allows in-situ visualization and structure determination of macromolecular complexes at sub-nanometre resolution. Cryogenic focused ion beam (cryo-FIB) micromachining is used to prepare a thin lamella-s
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Contributed
3 records found
The Nobel Prize in Chemistry in 2017 went to the development of cryo-electron microscopy, which contributed to the discovery of new cells and viruses, the diagnosis of unknown diseases, the mapping of human genomes and the manufacturing of semiconductors. Cryo Correlative light a
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Plunge freezing is used as a sample preparation method to freeze biological samples in a vitrified thin water layer of <500nm. Typically sample is loaded on thin (<10nm) carbon membranes of a copper mesh grid. The vitrified biological sample is imaged using high resolution cryoge
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