The increasing demand for precise light control in biomedical research, especially in fields like optogenetics and liquid crystal elastomers (LCEs), has exposed the limitations of traditional light sources regarding precision, controllability, and size. To address these challenges, this research focuses on developing miniaturized LED matrix systems capable of producing high-precision, high-power customizable light patterns essential for complex biological experiments. Two mini LED matrix systems—a 48x32 matrix and a larger 160x160 matrix—were designed and developed to achieve precise modulation of light intensity and patterns. These systems were tailored to meet the rigorous requirements of optogenetic experiments, which demand precise control over cellular activities, and to explore the potential applications in light-responsive LCEs. Controlling the light-responsive behavior of LCEs is critical for advancements in soft robotics, artificial muscles, and other applications requiring adjustable light sources. The 48x32 mini LED matrix system was successfully applied to control cardiac activity through optogenetics, demonstrating its ability to modulate light for inducing and terminating arrhythmias precisely. Additionally, its integration into LCE experiments showed promise for broader biomedical applications. Building on this success, the development of a larger 160x160 mini LED matrix system extended the technology’s applicability, supporting larger-scale experimental setups and human-sized models. By providing innovative solutions to the challenges of precise light control in biomedical research, this work opens new possibilities for experimental and clinical applications, offering significant contributions to the field.