Kv
K.P. van Remundt
info
Please Note
<p>This page displays the records of the person named above and is not linked to a unique person identifier. This record may need to be merged to a profile.</p>
2 records found
1
Geneflow
Localised Viral Vector Release for Optogenetic Application
In this thesis, applying viral vectors to the heart will be researched. Optogenetics is a quickly emerging field within microbiology. With a quickly emerging field of research, solutions for applying the viral vectors in a safe, quick and easy way are required.
The current method of genepainting requires a surgical procedure. To apply viral vectors into tissue, the two other main methods of applying viral vectors are encapsulation and microneedling. Encapsulation has been shown in literature to be too novel, with some disadvantages. The decision was made to make microneedles based on the material PVA.
For the production of PVA needles, moulds were made, which are shaped similarly to the design. For this mould production, 3D printing and laser cutting were explored, out of which the 3D printed moulds showed the most reliable characteristics.
The material PVA has been made with two different production processes and materials. The first one is solely PVA, which was solidified by repeated freeze-thaw cycles, and the second one is a mixture of PVA and sucrose solidified by drying the material. The first production has been used to show that the microneedles do penetrate heart tissue; it can be used in combination with viruses, and it has a reliable production method. The second recipe involves mixing sucrose in the PVA, which has been shown to dissolve and decrease in size.
The conclusion shows that for all the aspects of localised viral vector application in the context of optogenetics, PVA microneedles are highly likely to be able to fulfil that role. This will be shown by looking at aspects such as penetration of the heart, solubility of the materials, expression rate after production, and more. ...
The current method of genepainting requires a surgical procedure. To apply viral vectors into tissue, the two other main methods of applying viral vectors are encapsulation and microneedling. Encapsulation has been shown in literature to be too novel, with some disadvantages. The decision was made to make microneedles based on the material PVA.
For the production of PVA needles, moulds were made, which are shaped similarly to the design. For this mould production, 3D printing and laser cutting were explored, out of which the 3D printed moulds showed the most reliable characteristics.
The material PVA has been made with two different production processes and materials. The first one is solely PVA, which was solidified by repeated freeze-thaw cycles, and the second one is a mixture of PVA and sucrose solidified by drying the material. The first production has been used to show that the microneedles do penetrate heart tissue; it can be used in combination with viruses, and it has a reliable production method. The second recipe involves mixing sucrose in the PVA, which has been shown to dissolve and decrease in size.
The conclusion shows that for all the aspects of localised viral vector application in the context of optogenetics, PVA microneedles are highly likely to be able to fulfil that role. This will be shown by looking at aspects such as penetration of the heart, solubility of the materials, expression rate after production, and more. ...
In this thesis, applying viral vectors to the heart will be researched. Optogenetics is a quickly emerging field within microbiology. With a quickly emerging field of research, solutions for applying the viral vectors in a safe, quick and easy way are required.
The current method of genepainting requires a surgical procedure. To apply viral vectors into tissue, the two other main methods of applying viral vectors are encapsulation and microneedling. Encapsulation has been shown in literature to be too novel, with some disadvantages. The decision was made to make microneedles based on the material PVA.
For the production of PVA needles, moulds were made, which are shaped similarly to the design. For this mould production, 3D printing and laser cutting were explored, out of which the 3D printed moulds showed the most reliable characteristics.
The material PVA has been made with two different production processes and materials. The first one is solely PVA, which was solidified by repeated freeze-thaw cycles, and the second one is a mixture of PVA and sucrose solidified by drying the material. The first production has been used to show that the microneedles do penetrate heart tissue; it can be used in combination with viruses, and it has a reliable production method. The second recipe involves mixing sucrose in the PVA, which has been shown to dissolve and decrease in size.
The conclusion shows that for all the aspects of localised viral vector application in the context of optogenetics, PVA microneedles are highly likely to be able to fulfil that role. This will be shown by looking at aspects such as penetration of the heart, solubility of the materials, expression rate after production, and more.
The current method of genepainting requires a surgical procedure. To apply viral vectors into tissue, the two other main methods of applying viral vectors are encapsulation and microneedling. Encapsulation has been shown in literature to be too novel, with some disadvantages. The decision was made to make microneedles based on the material PVA.
For the production of PVA needles, moulds were made, which are shaped similarly to the design. For this mould production, 3D printing and laser cutting were explored, out of which the 3D printed moulds showed the most reliable characteristics.
The material PVA has been made with two different production processes and materials. The first one is solely PVA, which was solidified by repeated freeze-thaw cycles, and the second one is a mixture of PVA and sucrose solidified by drying the material. The first production has been used to show that the microneedles do penetrate heart tissue; it can be used in combination with viruses, and it has a reliable production method. The second recipe involves mixing sucrose in the PVA, which has been shown to dissolve and decrease in size.
The conclusion shows that for all the aspects of localised viral vector application in the context of optogenetics, PVA microneedles are highly likely to be able to fulfil that role. This will be shown by looking at aspects such as penetration of the heart, solubility of the materials, expression rate after production, and more.
Bluetooth Ultrasimple Gamepad
Design of a wireless cooperative gaming device
Bachelor thesis
(2021)
-
J.W. van der Linden, F.T. van der Heijde, K.P. van Remundt, R.E. Kooij, I.E. Lager, E.W. Bol
The design, production and testing of the Bluetooth Ultrasimple Gamepad, or BUG for short, is laid out in this report. In the current market, most of the controllers available are multi-button controllers with analogue sticks. These controllers are expensive and can not be used for many web browser games which rely on a keyboard input to work. In order for these controllers to work, a video game or self developed app can be used, but this can be difficult or expensive for many users. In the implementation of the BUG, the controller was made to be used by a single hand, having a clear ”main” button, an intuitive reconfiguration scheme and to have Bluetooth connection which is present in a lot of laptops and PCs currently in use. With this approach, the user can have a cheap, simple and comfortable alternative to existing gamepads. It can be stated
that, although not for everybody, the BUG is a good and fun alternative for users. This can be seen because the general user experience is rated at 5 out of 5 points by 50% of the participants, 4 out of 5 by 46.7% of the participants and only 1, or 3.3% of the participants has given a rating of 2 out of 5. ...
that, although not for everybody, the BUG is a good and fun alternative for users. This can be seen because the general user experience is rated at 5 out of 5 points by 50% of the participants, 4 out of 5 by 46.7% of the participants and only 1, or 3.3% of the participants has given a rating of 2 out of 5. ...
The design, production and testing of the Bluetooth Ultrasimple Gamepad, or BUG for short, is laid out in this report. In the current market, most of the controllers available are multi-button controllers with analogue sticks. These controllers are expensive and can not be used for many web browser games which rely on a keyboard input to work. In order for these controllers to work, a video game or self developed app can be used, but this can be difficult or expensive for many users. In the implementation of the BUG, the controller was made to be used by a single hand, having a clear ”main” button, an intuitive reconfiguration scheme and to have Bluetooth connection which is present in a lot of laptops and PCs currently in use. With this approach, the user can have a cheap, simple and comfortable alternative to existing gamepads. It can be stated
that, although not for everybody, the BUG is a good and fun alternative for users. This can be seen because the general user experience is rated at 5 out of 5 points by 50% of the participants, 4 out of 5 by 46.7% of the participants and only 1, or 3.3% of the participants has given a rating of 2 out of 5.
that, although not for everybody, the BUG is a good and fun alternative for users. This can be seen because the general user experience is rated at 5 out of 5 points by 50% of the participants, 4 out of 5 by 46.7% of the participants and only 1, or 3.3% of the participants has given a rating of 2 out of 5.