Nutrient limitation has been proposed as a biofouling control strategy for membrane systems. However, the impact of permeation on biofilm development under phosphorus-limited and enriched conditions is poorly understood. This study analyzed biofilm development in membrane fouling simulators (MFSs) with and without permeation supplied with water varying dosed phosphorus concentrations (0 and 25 µg P·L⁻¹). The MFSs operated under permeation conditions were run at a constant flux of 15.6 L·m^2·h−1 for 4.7 days. Feed channel pressure drop, transmembrane pressure, and flux were used as performance indicators. Optical coherence tomography (OCT) images and biomass quantification were used to analyze the developed biofilms. The total phosphorus concentration that accumulated on the membrane and spacer was quantified by using microwave digestion and inductively coupled plasma atomic emission spectroscopy (ICP-OES). Results show that permeation impacts biofilm development depending on nutrient condition with a stronger impact at low P concentration (pressure drop increase: 282%; flux decline: 11%) compared to a higher P condition (pressure drop increase: 206%; flux decline: 2%). The biofilm that developed at 0 µg P·L⁻¹ under permeation conditions resulted in a higher performance decline due to biofilm localization and spread in the MFS. A thicker biofilm developed on the membrane for biofilms grown at 0 µg P·L⁻¹ under permeation conditions, causing a stronger effect on flux decline (11%) compared to non-permeation conditions (5%). The difference in the biofilm thickness on the membrane was attributed to a higher phosphorus concentration in the membrane biofilm under permeation conditions. Permeation has an impact on biofilm development and, therefore, should not be excluded in biofouling studies.

Biofouling is a problem that hinders sustainable membrane-based desalination and the stratification of bacterial populations over the biofilm’s height is suggested to compromise the efficiency of cleaning strategies. Some studies reported a base biofilm layer attached to the membrane that is harder to remove. Previous research suggested limiting the concentration of phosphorus in the feed water as a biofouling control strategy. However, the existence of bacterial communities growing under phosphorus-limiting conditions and communities remaining after cleaning is unknown. This study analyzes the bacterial communities developed in biofilms grown in membrane fouling simula-tors (MFSs) supplied with water with three dosed phosphorus conditions at a constant biodegradable carbon concentration. After biofilm development, biofilm was removed using forward flushing (an easy-to-implement and environmentally friendly method) by increasing the crossflow velocity for one hour. We demonstrate that small changes in phosphorus concentration in the feed water led to (i) different microbial compositions and (ii) different bacterial-cells-to-EPS ratios, while (iii) similar bacterial biofilm populations remained after forward flushing, suggesting a homogenous bacterial community composition along the biofilm height. This study represents an exciting advance towards greener desalination by applying non-expensive physical cleaning methods while manipulating feed water nutrient conditions to prolong membrane system performance and enhance membrane cleanability.

A critical problem in seawater reverse osmosis (RO) filtration processes is biofilm accumulation, which reduces system performance and increases energy requirements. As a result, membrane systems need to be periodically cleaned by combining chemical and physical protocols. Nutrient limitation in the feed water is a strategy to control biofilm formation, lengthening stable membrane system performance. However, the cleanability of biofilms developed under various feed water nutrient conditions is not well understood. This study analyzes the removal efficiency of biofilms grown in membrane fouling simulators (MFSs) supplied with water varying in phosphorus concentrations (3 and 6 μg P·L^-1 and with constant biodegradable carbon concentration) by applying hydraulic cleaning after a defined 140% increase in the feed channel pressure drop, through increasing the cross-flow velocity from 0.18 m s^-1 to 0.35 m s^-1 for 1 h. The two phosphorus concentrations (3 and 6 μg P·L^-1) simulate the RO feed water without and with the addition of a phosphorus-based antiscalant, respectively, and were chosen based on measurements at a full-scale seawater RO desalination plant. Biomass quantification parameters performed after membrane autopsies such as total cell count, adenosine triphosphate, total organic carbon, and extracellular polymeric substances were used along with feed channel pressure drop measurements to evaluate biofilm removal efficiency. The outlet water during hydraulic cleaning (1 h) was collected and characterized as well. Optical coherence tomography images were taken before and after hydraulic cleaning for visualization of biofilm morphology. Biofilms grown at 3 μg P·L^-1 had an enhanced hydraulic cleanability compared to biofilms grown at 6 μg P·L^-1. The higher detachment for biofilms grown at a lower phosphorus concentration was explained by more soluble polymers in the EPS, resulting in a lower biofilm cohesive and adhesive strength. This study confirms that manipulating the feed water nutrient composition can engineer a biofilm that is easier to remove, shifting research focus towards biofilm engineering and more sustainable cleaning strategies.

Nutrient limitation is a biofouling control strategy in reverse osmosis (RO) membrane systems. In seawater, the assimilable organic carbon content available for bacterial growth ranges from about 50 to 400 μg C·L⁻¹, while the phosphorus concentration ranges from 3 to 11 μg P·L⁻¹. Several studies monitored biofouling development, limiting either carbon or phosphorus. The effect of carbon to phosphorus ratio and the restriction of both nutrients on membrane system performance have not yet been investigated. This study examines the impact of reduced phosphorus concentration (from 25 μg P·L⁻¹ and 3 μg P·L⁻¹, to a low concentration of ≤0.3 μg P·L⁻¹), combined with two different carbon concentrations (250 C L⁻¹ and 30 μg C·L⁻¹), on biofilm development in an RO system. Feed channel pressure drop was measured to determine the effect of the developed biofilm on system performance. The morphology of the accumulated biomass for both carbon concentrations was characterized by optical coherence tomography (OCT) and the biomass amount and composition was quantified by measuring total organic carbon (TOC), adenosine triphosphate (ATP), total cell counts (TCC), and extracellular polymeric substances (EPS) concentration for the developed biofilms under phosphorus restricted (P-restricted) and dosed (P-dosed) conditions. For both carbon concentrations, P-restricted conditions (≤0.3 μg P·L⁻¹) limited bacterial growth (lower values of ATP, TCC). A faster pressure drop increase was observed for P-restricted conditions compared to P-dosed conditions when 250 μg C·L⁻¹ was dosed. This faster pressure drop increase can be explained by a higher area covered by biofilm in the flow channel and a higher amount of produced EPS. Conversely, a slower pressure drop increase was observed for P-restricted conditions compared to P-dosed conditions when 30 μg C·L⁻¹ was dosed. Results of this study demonstrate that P-limitation delayed biofilm formation effectively when combined with low assimilable organic carbon concentration and thereby, lengthening the overall membrane system performance.

Biofouling severely impacts operational performance of membrane systems increasing the cost of water production. Understanding the effect of critical parameters of feed water such as biodegradable substrate concentration on the developed biofilm characteristics enables development of more effective biofouling control strategies. In this study, the effect of substrate concentration on the biofilm characteristics was examined using membrane fouling simulators (MFSs). A feed channel pressure drop (PD) increase of 200 mbar was used as a benchmark to study the developed biofilm. The amount and characteristics of the formed biofilm were analysed in relation to membrane performance indicators: feed channel pressure drop and permeate flux. The effect of the characteristics of the biofilm developed at three substrate concentrations on the removal efficiency of the different biofilms was evaluated applying acid/base cleaning. Results showed that a higher feed water substrate concentration caused a higher biomass amount, a faster PD increase, but a lower permeate flux decline. The permeate flux decline was affected by the spatial location and the physical characteristics of the biofilm rather than the total amount of biofilm. The slower growing biofilm developed at the lowest substrate concentration was harder to remove by NaOH/HCl cleanings than the biofilm developed at the higher substrate concentrations. Effective biofilm removal is essential to prevent a fast biofilm regrowth after cleaning. While substrate limitation is a generally accepted biofouling control strategy delaying biofouling, development of advanced cleaning methods to remove biofilms formed under substrate limited conditions is of paramount importance.