Single‐Molecule Switching

Fluorescence Polarization Control for On–Off Switching of Single Molecules at Cryogenic Temperatures

Journal article (2018)

Authors

C.N. Hulleman ImPhys/Quantitative Imaging
Maximiliaan Huisman University of Massachusetts Medical School
R.J. Moerland ImPhys/Quantitative Imaging
David Grunwald RNA Therapeutics Institute, University of Massachusetts Medical School
S. Stallinga ImPhys/Imaging Physics
B. Rieger ImPhys/Quantitative Imaging
Research Group
ImPhys/Quantitative Imaging
Copyright: © 2018 C.N. Hulleman, Maximiliaan Huisman, R.J. Moerland, David Grünwald, S. Stallinga, B. Rieger
DOI: https://doi.org/10.1002/smtd.201870044
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Published Date

2018

Language

English

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Research Group

ImPhys/Quantitative Imaging

Abstract

In article number 1700323, a sparsity‐inducing scheme based on widefield stimulated emission depletion, which requires stringent control of the polarization state of both the excitation and depletion laser, is introduced by Bernd Rieger and co‐workers for superresolution fluorescence microscopy. The ideal state involves excitation and depletion lasers with linear polarizations, orthogonally oriented in the sample plane. This state is obtained from linearly polarized light from the laser, which is made elliptically polarized before reflecting off the dichroic mirror.

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