The gRAMP CRISPR-Cas effector is an RNA endonuclease complexed with a caspase-like peptidase

Journal article (2021)

Authors

S.P.B. van Beljouw BN/Stan Brouns Lab - AS
A.C. van Eijkeren-Haagsma Kavli institute of nanoscience Delft, BN/Technici en Analisten
A. Rodriguez Molina BT/Biocatalysis - AS , Kavli institute of nanoscience Delft
D.F. van den Berg External organisation, Kavli institute of nanoscience Delft
J.N.A. Vink Kavli institute of nanoscience Delft, BN/Stan Brouns Lab - AS
S.J.J. Brouns BN/Stan Brouns Lab - AS , Kavli institute of nanoscience Delft
Research Group
BN/Stan Brouns Lab (AS) (TU Delft)
Copyright: © 2021 S.P.B. van Beljouw, A.C. van Eijkeren-Haagsma, A. Rodriguez Molina, D.F. van den Berg, J.N.A. Vink, S.J.J. Brouns
DOI
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https://doi.org/10.1126/science.abk2718
More Info
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Published Date

2021

Language

English

Reuse Rights

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Faculty

Applied Sciences

Department

BN/Bionanoscience

Research Group

BN/Stan Brouns Lab

Abstract

Type III CRISPR-Cas immunity is widespread in prokaryotes and is generally mediated by multisubunit effector complexes. These complexes recognize complementary viral transcripts and can activate ancillary immune proteins. Here, we describe a type III-E effector from Candidatus “Scalindua brodae” (Sb-gRAMP), which is natively encoded by a single gene with several type III domains fused together. This effector uses CRISPR RNA to guide target RNA recognition and cleaves single-stranded RNA at two defined positions six nucleotides apart. Sb-gRAMP physically combines with the caspase-like TPR-CHAT peptidase to form the CRISPR-guided caspase (Craspase) complex, suggesting a potential mechanism of target RNA-induced protease activity to gain viral immunity.