Approaches for bacteriophage genome engineering
M.S. Mahler (TU Delft - BN/Stan Brouns Lab, University of Otago)
Ana Rita Martins Costa (TU Delft - BN/Stan Brouns Lab, Kavli institute of nanoscience Delft)
S.P.B. van Beljouw (Kavli institute of nanoscience Delft, TU Delft - BN/Stan Brouns Lab)
Peter C. Fineran (University of Otago)
Stan J.J. Brouns (TU Delft - BN/Stan Brouns Lab, Kavli institute of nanoscience Delft)
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Abstract
In recent years, bacteriophage research has been boosted by a rising interest in using phage therapy to treat antibiotic-resistant bacterial infections. In addition, there is a desire to use phages and their unique proteins for specific biocontrol applications and diagnostics. However, the ability to manipulate phage genomes to understand and control gene functions, or alter phage properties such as host range, has remained challenging due to a lack of universal selectable markers. Here, we discuss the state-of-the-art techniques to engineer and select desired phage genomes using advances in cell-free methodologies and clustered regularly interspaced short palindromic repeats-CRISPR associated protein (CRISPR-Cas) counter-selection approaches.
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