Localization microscopy at doubled precision with patterned illumination
Jelmer Cnossen (TU Delft - ImPhys/Quantitative Imaging, TU Delft - Team Raf Van de Plas)
Taylor Hinsdale (TU Delft - ImPhys/Quantitative Imaging)
Rasmus Thorsen (TU Delft - ImPhys/Quantitative Imaging)
Marijn Siemons (Universiteit Utrecht)
Florian Schueder (Ludwig Maximilians University, Max Planck Institute of Biochemistry)
Ralf Jungmann (Max Planck Institute of Biochemistry, Ludwig Maximilians University)
Carlas S. Smith (TU Delft - Team Raf Van de Plas, University of Oxford, TU Delft - ImPhys/Quantitative Imaging)
Bernd Rieger (TU Delft - ImPhys/Quantitative Imaging)
Sjoerd Stallinga (TU Delft - ImPhys/Imaging Physics)
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Abstract
MINFLUX offers a breakthrough in single molecule localization precision, but is limited in field of view. Here we combine centroid estimation and illumination pattern induced photon count variations in a conventional widefield imaging setup to extract position information over a typical micrometer-sized field of view. We show a near two-fold improvement in precision over standard localization with the same photon count on DNA-origami nanostructures and tubulin in cells, using DNA-PAINT and STORM imaging.