Cryogenic 4Pi Single Molecule Localization Microscopy (Cryo-4Pi-SMLM)
A 3D nanometer imaging technique
Q. Li (TU Delft - ImPhys/Stallinga group, TU Delft - ImPhys/Rieger group)
S Stallinga – Promotor (TU Delft - ImPhys/Stallinga group)
B Rieger – Promotor (TU Delft - ImPhys/Rieger group)
More Info
expand_more
Other than for strictly personal use, it is not permitted to download, forward or distribute the text or part of it, without the consent of the author(s) and/or copyright holder(s), unless the work is under an open content license such as Creative Commons.
Abstract
Single molecule localization microscopy (SMLM) surpasses the diffraction limit by sequentially imaging individual fluorescent molecules. By incorporating 4Pi detection to capture self-interference from the fluorescence emitters via two objective lenses, 4Pi-SMLM achieves isotropic sub 5 nm resolution in three dimensions (3D). This thesis details the design, construction, and evaluation of a Cryo-4Pi-SMLM system, aiming to enable subcellular 3D fluorescence imaging in native states with isotropic localization precision under cryogenic conditions, providing a 3D correlative light and electron microscopy solution...
help_outline