Natronomicrosphaera hydrolytica, gen. nov., sp. nov., a first representative of the phylum Planctomycetota from soda lakes
DImitry Y. Sorokin (TU Delft - BT/Environmental Biotechnology, Russian Academy of Sciences)
Alexander Y. Merkel (Russian Academy of Sciences)
Nicole J. Bale (NIOZ Royal Netherlands Institute for Sea Research)
Michel Koenen (NIOZ Royal Netherlands Institute for Sea Research)
Jaap S. Sinninghe Damste (NIOZ Royal Netherlands Institute for Sea Research)
Laura Marturano (Institute of Polar Sciences, ISP-CNR)
Enzo Messina (Institute of Polar Sciences, ISP-CNR)
Violetta La Cono (Institute of Polar Sciences, ISP-CNR)
Michail M. Yakimov (Institute of Polar Sciences, ISP-CNR)
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Abstract
Despite intensive microbiological characterization of soda lake microbial communities, no culturable representatives from the phylum Planctomycetota have been isolated from these haloalkaline habitats. In the context of studying polysaccharide utilization by soda lake microbial communities, we used polysaccharide hyaluronic acid as enrichment substrate at aerobic, moderate haloalkaline conditions (1 M total Na+, pH 9.5). This resulted in a
selective enrichment and isolation in pure culture of a bacterial strain AB-hyl4 belonging to Planctomycetota. The cells are tiny motile cocci growing in large aggregates, with the Gram-negative type of ultrastructure and producing a yellow pigment. This obligate aerobic saccharolytic heterotroph has an extremely narrow growth substrate range including, besides hyaluronic acid, melezitose and glycerol. The membrane lipids consist of phosphatidylcholine and two types of neutral lipids, including hopanoids and monounsaturated C17 and C19 hydrocarbons. Phylogenomic analysis placed the isolate into the family Phycisphaeraceae, class Phycisphaerae, as a new genus-level lineage. Its genome contained a gene encoding a polysaccharide lyase from the PL8 family which is probably responsible for the degradation of hyaluronic acid to a dimer, followed by its transport and hydrolysis into monomers in periplasm and final glycolytic degradation in cytoplasm. On the basis of distinct phenotypic and genomic properties, strain AB-hyl4T (DSM 117794 = UQM 41914) is proposed to be classified as
Natronomicrosphaera hydrolytica gen. nov., sp. nov.
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