Towards preparative peroxygenase-catalyzed oxyfunctionalization reactions in organic media

Journal article (2016)

Authors

E. Fernandez Fueyo BT/Biocatalysis - AS
Y. Ni BT/Biocatalysis - AS
A. Gomez Baraibar BT/Biocatalysis - AS
Miguel Alcalde University of the Balearic Islands
F. Hollmann BT/Biocatalysis - AS
Research Group
BT/Biocatalysis (AS) (TU Delft)
Copyright: © 2016 E. Fernandez Fueyo, Y. Ni, A. Gomez Baraibar, Miguel Alcalde, L.M. van Langen, F. Hollmann
DOI
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https://doi.org/10.1016/j.molcatb.2016.09.013
Biocatalysis Hydroxylation Non-aqueous reaction media Oxyfunctionalization Peroxygenase
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Published Date

2016

Language

English

Faculty

Applied Sciences

Department

BT/Biotechnologie

Research Group

BT/Biocatalysis

Abstract

The peroxygenase from Agrocybe aegerita (AaeUPO) has been evaluated for stereoselective oxyfunctionalization chemistry under non-aqueous reaction conditions. The stereoselective hydroxylation of ethylbenzene to (R)-1-phenylethanol was performed in neat substrate as reaction medium together with the immobilized biocatalyst and tertBuOOH as oxidant. Stability and activity issues still have to be addressed. Nevertheless, gram-scale production of enantiopure (R)-1-phenylethanol was achieved with respectable 90,000 turnovers of the biocatalyst.