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A Photoenzymatic NADH Regeneration System

Journal Article (2018)
Author(s)

G.T. Höfler (TU Delft - BT/Biocatalysis)

Elena Fueyo (TU Delft - BN/Greg Bokinsky Lab)

M. Pešić (TU Delft - BT/Biocatalysis)

Sabry H.H. Younes (TU Delft - BT/Biocatalysis)

Eun Gyu Choi (Ulsan National Institute of Science and Technology)

Yong H. Kim (Ulsan National Institute of Science and Technology)

Vlada B. Urlacher (Universität Düsseldorf)

IWCE Arends (TU Delft - BT/Biotechnologie)

Frank Hollmann (TU Delft - BT/Biocatalysis)

Research Group
BT/Biocatalysis
Copyright
© 2018 G.T. Höfler, E. Fernandez Fueyo, M. Pesic, S.H.H. Younes, Eun Gyu Choi, Yong H. Kim, Vlada B. Urlacher, I.W.C.E. Arends, F. Hollmann
DOI related publication
https://doi.org/10.1002/cbic.201800530
More Info
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Publication Year
2018
Language
English
Copyright
© 2018 G.T. Höfler, E. Fernandez Fueyo, M. Pesic, S.H.H. Younes, Eun Gyu Choi, Yong H. Kim, Vlada B. Urlacher, I.W.C.E. Arends, F. Hollmann
Research Group
BT/Biocatalysis
Volume number
19
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Abstract

A photoenzymatic NADH regeneration system was established. The combination of deazariboflavin as a photocatalyst with putidaredoxin reductase enabled the selective reduction of NAD+ into the enzyme-active 1,4-NADH to promote an alcohol dehydrogenase catalysed stereospecific reduction reaction. The catalytic turnover of all the reaction components was demonstrated. Factors influencing the efficiency of the overall system were identified.