A Photoenzymatic NADH Regeneration System
Georg T. Höfler (TU Delft - Applied Sciences)
Elena Fernández-Fueyo (TU Delft - Applied Sciences)
Milja Pesic (TU Delft - Applied Sciences)
Sabry H. Younes (TU Delft - Applied Sciences)
Eun Gyu Choi (Ulsan National Institute of Science and Technology)
Yong H. Kim (Ulsan National Institute of Science and Technology)
Vlada B. Urlacher (Universität Düsseldorf)
Isabel W.C.E. Arends (TU Delft - Applied Sciences)
Frank Hollmann (TU Delft - Applied Sciences)
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Abstract
A photoenzymatic NADH regeneration system was established. The combination of deazariboflavin as a photocatalyst with putidaredoxin reductase enabled the selective reduction of NAD+ into the enzyme-active 1,4-NADH to promote an alcohol dehydrogenase catalysed stereospecific reduction reaction. The catalytic turnover of all the reaction components was demonstrated. Factors influencing the efficiency of the overall system were identified.
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