CRISPR-controlled proteases
S.P.B. van Beljouw (Kavli institute of nanoscience Delft, TU Delft - BN/Stan Brouns Lab)
Stan J. J. Brouns (Kavli institute of nanoscience Delft, TU Delft - BN/Stan Brouns Lab)
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Abstract
With the discovery of CRISPR-controlled proteases, CRISPR–Cas has moved beyond mere nucleic acid targeting into the territory of targeted protein cleavage. Here, we review the understanding of Craspase, the best-studied member of the growing CRISPR RNA-guided protease family. We recollect the original bioinformatic prediction and early experimental characterizations; evaluate some of the mechanistic structural intricacies and emerging biotechnology; discuss open questions and unexplained mysteries; and indicate future directions for the rapidly moving field of the CRISPR proteases.
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