A multiwell plate Organ-on-Chip (OOC) device for in-vitro cell culture stimulation and monitoring
N. Gaio (TU Delft - Electronic Components, Technology and Materials)
A. Waafi (Student TU Delft)
M.L.H. Vlaming (Ncardia)
E. Boschman (Boschman Technologies)
P. Dijkstra (Philips Innovation Services)
O. Nacken (Ncardia)
S.R. Braam (Ncardia)
C. Boucsein (Multi Channel Systems GmbH)
P. M. Sarro (TU Delft - Electronic Components, Technology and Materials)
R. Dekker (TU Delft - Electronic Components, Technology and Materials)
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Abstract
This work presents the first multi-well plate that allows for simultaneous mechanical stimulation and electrical monitoring of multiple in-vitro cell cultures in parallel. Each well of the plate is equipped with an Organ-on-Chip (OOC) device consisting of a stretchable micro-electrode array (MEA). For the first time, a film assisted molding (FAM) process was employed to embed an OOC into a multi well plate format packaging. The functionality of the MEA in the device was assessed with electrochemical impedance spectroscopy. Moreover, the biocompatibility of the plate was demonstrated with cardiomyocytes derived from human induced pluripotent stem cells (iPSC) cultured in the wells.